摘要
目的研究甘草次酸(glycyrrhetinic acid,GA)对培养的兔晶状体上皮细胞(rabbit lens epithelial cells,RLECs)增殖的影响及其机理。方法首先进行RLECs的体外培养,取第3代的RLECs分别加入不同浓度的GA和20 mg/L的MMC处理,观察细胞形态学变化、免疫细胞化学方法——脱氧核糖核苷酸末端转移酶介导的缺口末端标记法(terminal-deoxynucleotidyl transferase mediated nick end labeling,TUNEL)进一步鉴别凋亡细胞,利用流式细胞仪(flow cytometer,FCM)对细胞凋亡率进行定量检测。结果GA对体外培养的RLECs的增殖有显著的影响,RLECs的凋亡率随着GA的浓度增加和时间的延长而增加。结论GA可以引起体外培养的RLECs凋亡,且对RLECs凋亡诱导作用呈剂量和时间依赖性。
Objective To study the effect and mechanisms of glycyrrhetinic acid (GA) on the proliferation of rabbit lens epithelial cells ( RLECs ) in vitro and to pmvid the theory reference for developing the drug of preventioning and curing posterior capsular opacification(PCO). Methods RLECs were cultured in vitro first and GA of different concentrations and 20mg/L MMC were added to the rabbit lens epithelial cells of third passage. We observed cells morphological changes with light microscope and identified the apoptosis with immunocytochemical method, terminal - deoxynucleotidyl transferase mediated nick end labeling(TUNEL} and used flow cytometry(FCM) as a quantitative method of evaluation of these effect. Results GA could significantly affect the proliferation of RLECs. The apoptosis rate of RLECs was increased with the increase of the GA concentrations and the longing of time. Conclusion GA can induce apoptosis of rabbit lens epithelial cells cultured in vitro. The apoptosis rate of RLECs was depended on the GA concentrations and the longing of time.
出处
《南华大学学报(医学版)》
2007年第2期181-185,共5页
Journal of Nanhua University(Medical Edition)
基金
湖南省教育厅资助项目(项目编号:03C413)