小儿肾病、乙肝患者血清CaM与抗CaM自身抗体的测定研究

Serum CaM and Anti-CaM autoantibody determination in children with pediatric kidney and HBV hepatitis diseases

目的研究小儿疾病患者血清中CaM含量和抗CaM自身抗体阳性检出水,探讨CaM及抗CaM抗体与临床小儿疾病的关系。方法优化实验反应条件,建立检测CaM和抗CaM抗体的ELISA测定方法,并将其初步应用于临床小儿疾病患者血清CaM和抗CaM自身抗体水平测定。结果优化后的ELISA对CaM和抗CaM自身抗体的实验结果特异、敏感、可靠。在CaM浓度为39 ng/ml-2500 ng/ml范围内,该法对CaM的测定具有良好的线性关系,相关系数(r)大于0.99。临床研究结果表明,各类小儿肾脏疾病血清CaM含量与抗CaM自身抗体均处在与正常对照组相同的水平。但在小儿乙型肝炎患者血清中,两者水平均显著高于正常对照组。25例被检者中分别有5例CaM水平升高,有6例为抗CaM自身抗体阳性,其中有5例为二者水平同时升高。结论小儿在感染乙型肝炎病毒的发病过程中,即伴有一定程度的CaM和抗CaM自身抗体水平升高,据此推测抗CaM抗体在继发引起小儿自身免疫性肝损害的过程中可能起一定作用。

Objective To investigate serum levels of CaM and Anti-CaM autoanfibody in pediatric kidney and HBV hepatitis diseases,and explore the relationship between levels of serum CaM,Anti-CaM auto-antibody and pediatric diseases.Methods Two types of ELISA method for serum CaM and anti-CaM autoantibody were established by optimizing experimental conditions,and preliminarily used for pediatric diseases research.Results The results of assays for serum CaM and Anti-CaM autoantibody were specific,sensitive and reliable.Under the circumstance of CaM concentrations range from 39ng/ml to 2500ng/ml, this assay for CaM provided with a good linear correlations（ r 〉 0.99）.In comparison with normal control,serum levels of CaM and anti-CaM autoantibody in various pediatric kidney diseases were no significant difference. But in pediatric HBV hepatitis, both of them were significantly increased than normal control.In 25 eases with HBV hepatitis,5 of them were higher levels of CaM,and 6 d them were positive with anti-CaM au- toantibody. Among these,5 cases were synchronously abnormal with higher levers of CaM and anti-CaM autoanfibody. Condusion There exists higher levels of senan CaM and anti-CaM autoantibody in pediatric HBV hepatitis, and suggests that anti-CaM autoanfibody will be a .key event in the development of autoimmune liver disease.