摘要
目的研究结膜上皮细胞体外培养的方法,并进一步探索无血清培养系统用于结膜上皮细胞培养的可行性。方法分别用含胎牛血清培养液和不含血清培养液进行组织块培养,观察两种培养方法在细胞形态、细胞生长情况和增殖及细胞分化方面的差异。结果无血清培养系统中结膜上皮细胞的增殖、克隆形成及细胞传代能力均较含血清培养系统高,而且具备结膜上皮细胞全部组织结构及功能特点。结论无血清培养系统的组织块培养法可为结膜上皮细胞的疾病、药理毒理研究及培养移植的结膜上皮细胞提供更为安全有效的细胞模型。
Objective To investigate the culturing teclmique for eonjunetival epithelial cells and to evaluate the facibility of serum-frce culture system on culturing conjunctival epithelial explants. Methods Rabbit conjunctival epithelial tissues were explanted in serum-free system. The morphology, gowth, proliferation and differentiation of outgrow cells were compared with those in serum-containing medium. Results The epithelial cells cultured in serum-free medium not only demonstrated a significant increase in proliferative,colony-form/ng efficiency and cell generations,but also have the normal structural and functional properties than those in serumcontaining medium. Conclusion This is a sermn-free culture system for developing a conjunctival epithelial explants with improved purify and proliferative, which are important for the basic research and tissue engineer of conjunctival epithelial ceils.
出处
《中国实验诊断学》
2007年第3期361-363,共3页
Chinese Journal of Laboratory Diagnosis
