人参皂苷超声水提取法的改进

Improvied Method of Conventional Ultrasonic Water Extraction of Ginsenosides

以人参总皂苷、人参皂苷-Rb1,-Rb2,-Rb3,-Rc,-Rd,-Re,-Rf,-Rg1和-Rg2等9种主要单体皂苷的收率为指标,利用超声波提取器提取,大孔吸附树脂法去除杂质,高效液相色谱法测定含量,比较稀醋酸提取法和常规超声水提取法的优劣.结果表明,稀醋酸提取法所得总皂苷和各单体皂苷收率均比常规超声水提法明显提高,t检验证明两种提取方法有显著的差异(P<0.001).稀醋酸提取法对常规超声水提法进行改进,此法收率高、纯度大、操作简便,适合工业化生产.

We established a diluted acetic acid extraction method and compared the yields of total ginsenosides and nine monoginsenosides （ ginsenoside -Rb1,-Rb2,-Rb3,-Re,-Rd,-Re,-R.f,-Rg1 and -Rg2 ） via our method with those via the conventional ultrasonic water extraction method, after total ginsenosides and nine monoginsenosides were measured with HPLC after purified by the macroporous resin method. The results show the diluted acetic acid extraction method is better than the conventional ultrasonic water extraction method, the total ginsenosides and monoginsenosides yields via the diluted acetic acid extraction method were significantly increased compared with those via the conventional ultrasonic water extraction （ P 〈 0.001 ）. The diluted acetic acid extraction method is a improved method of the conventional ultrasonic water extraction of ginsenosides and a new method, which has a high extraction yield and the operation is simple, and meets the need of industrialized manufacture.