摘要
由本实验室产胆固醇氧化酶的菌株类芽胞杆菌作为出发菌株,经过紫外+LiCl、紫外+光敏试剂(8—mop)物理化学诱变处理,最后得到一株突变株XUM-10,并对其进行发酵条件的优化,最终酶活为221.20U/L,其酶活为出发菌株的2.5倍。
The mutation swain was obtained from Paenibacillus sp, X534 of our lab, and was mutagcnized with Ullraviolet＋LiCl and Ullraviolct＋8-mop, giving XUM-10 strain. The fermentation conditions of XUM-10 strain were also studied. Results showed that the enzyme activity was 221.20U/L, which was 2.5 times higher than parent strain.
出处
《现代食品科技》
EI
CAS
2007年第3期7-10,共4页
Modern Food Science and Technology
关键词
类芽胞杆菌
胆固醇氧化酶
诱变选育
Paenibacillus sp.
cholesterol oxidase
mutation and breeding
