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胆固醇氧化酶高产菌类芽胞杆菌的诱变选育 被引量:2

Mutation and Breeding of Paenibacillus sp. with high Cholesterol Oxidase Activity
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摘要 由本实验室产胆固醇氧化酶的菌株类芽胞杆菌作为出发菌株,经过紫外+LiCl、紫外+光敏试剂(8—mop)物理化学诱变处理,最后得到一株突变株XUM-10,并对其进行发酵条件的优化,最终酶活为221.20U/L,其酶活为出发菌株的2.5倍。 The mutation swain was obtained from Paenibacillus sp, X534 of our lab, and was mutagcnized with Ullraviolet+LiCl and Ullraviolct+8-mop, giving XUM-10 strain. The fermentation conditions of XUM-10 strain were also studied. Results showed that the enzyme activity was 221.20U/L, which was 2.5 times higher than parent strain.
出处 《现代食品科技》 EI CAS 2007年第3期7-10,共4页 Modern Food Science and Technology
关键词 类芽胞杆菌 胆固醇氧化酶 诱变选育 Paenibacillus sp. cholesterol oxidase mutation and breeding
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参考文献8

  • 1[1]National Research Council,Diet and health implications for reducing chronic desease risk,National Academy Press Washington.DC.1989
  • 2[2]Allain C Poon L S,Chan C S G,Richmond W Fu P C.Enzymatic Determination of Total Serum Cholesterol.Clin Chem,1974,20:470-475
  • 3[3]Somkuti G A,Solaiman D K&Steinberg D H.Native Promoter-plasmid vector system for heterologous cholesterol oxidase synthesis in Streptococcus thermophilus.Plasmid,1995,33:7-14
  • 4[4]Murooka Y,Choi K,Molnar I,Dziadek J,Cho H,Nomura N&Yamashita M.Recent Advances in Studies on Streptomyces Cholesterol Oxidase and Gene Clusters Involved in Steroid Catabolism in Prokaryotes.Actinomycetol,1996,10:1-11
  • 5[5]Stevens VL,XwT&Lambeth J D.Cholesterol pools in rat adrenal mitochondria:use of cholesterol oxidase to infer a complex pool structure.Endocrinology,1992,130:1557-1563
  • 6[6]Ghoshroy KB,ZhuW &Sampson N S.Investigation of Membrane Disruption in the Reaction Catalyzed by Cholesterol Oxidase.Biochemistry,1997,36:6133-6140
  • 7季文明,陈毅力,张和春,王武.比色法测定胆固醇氧化酶酶活[J].无锡轻工大学学报(食品与生物技术),2000,19(3):251-254. 被引量:36
  • 8赵芳,梁海秋,杨辉,王发合,周河治.高活性胆固醇氧化酶产生菌出发菌株的筛选[J].现代食品科技,2006,22(2):67-69. 被引量:2

二级参考文献5

  • 1[2]Volker F Wendishch,Albert A DE Graat,Hermann Sahm,et al.Quantitative determination of metabolic fluxes during coutilization of two carbon sources:comparative analysis with Corynebacterium glutamicum during growth on acetate and glucose[J].Journal of Biotechnology.2000,3088-3096
  • 2[3]Purcell John D,Greenplate John T,Jennings Michael G.et al.Cholesterol Oxidase:A Potent Larvae[J].Biochem.Biophys.Res.Commum.1993,196(3):1406-1413
  • 3[4]Suzukl Kunio.4-Chotesten-3-one for the Control of Obeslty[P].PCT Int.Appl.Wo 93(12) 718
  • 4[6]许哓文,扬万龙和沈含熙编著.定量分析.天津:南开大学出版社,1996
  • 5颜思旭,酶催化动力学原理与方法,1987年

共引文献36

同被引文献26

  • 1桑吉,王龙刚,李忠琴,王武.胆固醇氧化酶高产菌株的复合诱变选育[J].食品工业科技,2006,27(6):87-89. 被引量:17
  • 2Charles CA,Poon LS,Chan CGS,et al.Enzymatic determination of total serum cholesterol.Clin Chem,1974,20(2):470-475.
  • 3Smith GA,Brooks JW.Cholesterol Oxidases:Properties and Application.Steroid Biochemistry,1976,7(9):705-713.
  • 4Lebovics VK,Magdolna A,Odon G.Enzymatic determination of cholesterol oxides.J Sci Food Agric,1996,71:22-26.
  • 5Gorge AS,Daniel K.Transfer and expression of a Streptomyces cholesterol oxidase gene in Streptococcus thermophilus.Appl Microbiol Biotechnol,1992,37:330-334.
  • 6Smith M,Sullivan C,Goodman N.Reactivity of milk cholesterol with bacterial cholesterol oxidases.J Agric Food Chem,1991,39:2158-2162.
  • 7Yazdia TM,Zahraeib M,Aghaepoura K,et al.Purification and partial characterization of a cholesterol oxidase from Streptomyces fradiae.Enzyme and Microbial Technology,2001,28(4):410-414.
  • 8Varma R,Nene S.Biosynthesis of cholesterol oxidase by Streptomyces Lavendulae NCIM 2421.Enzyme and Microbial Technology,2003,33(2):286-291.
  • 9Wang YQ,Li Qs.Natural Carotenoids Research,Production,Application.Beijing:China Medical Publishing House,1997.
  • 10Allain C C,Poon L S, Chan C S G, Richmond W, Fu P C. 1974. Enzymatic determination of total serum cholesterol [J]. Clinical Chemistry, 20: 470-475.

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