乙醇对大鼠胃底5-羟色胺2B受体表达水平影响的研究

Effect of alcohol on the expression level of 5-hydroxytryptamine 2B receptor in rat stomach fundus

目的:检测口服乙醇后大鼠胃底5-羟色胺2B(5-HT2B)受体表达水平的变化,探讨乙醇对大鼠胃底收缩的影响。方法:提取实验处理组及对照组总RNA,用半定量RT-PCR法检测5-HT2B受体mRNA表达水平。扩增大鼠5-HT2B受体基因,扩增产物用琼脂糖凝胶电泳检测,并用GDS8000凝胶成像及分析系统分析电泳条带密度。结果:大鼠胃底5-HT2B受体表达水平在给予乙醇2h后增高,与对照组比较有显著性差异(P<0.05)。结论:乙醇可引起大鼠胃底5-HT2B受体表达水平增高。

Objective： To study the expression level of 5-hydroxytryptamine 2B （5-HT2B） receptor in rat stomach fundus after oral administration of alcohol,and to explore the effect of alcohol on the contraction of rat stomach fundus. Methods： Ten Wistar rats were randomly divided into treatment group and control group,in which the rats were orally administered alcohol and normal saline,respectively. Total RNA was extracted,and the expression level of 5-HT2B receptor mRNA was determined by semi-quantitative reverse transcriptase polymerase chain reaction. The PCR products were detected with agarose electmphoresis,and the densitometry analysis was performed by using GDS-800 system. Results：The expression level of 5-HT2B receptor in treatment group was significantly higher than that in control group 2 hours after the administration of alcohol （P 〈 0.05 ）. Condusion： Alcohol could increase the expression level of 5-HT2B receptor in rat stomach fundus.