成人骨髓间充质干细胞对K562细胞生长的影响

Effects of adult human bone marrow mesenchymal stem cells on the growth of K562 cells

目的:研究成人骨髓间充质干细胞(MSCs)对K562细胞生长的影响。方法:从成人骨髓中分离、培养MSCs,并通过其形态的均一性及流式细胞术检测其表面标志以鉴定其纯度,观察其电镜下的超微结构。将分离培养的MSCs置于24孔板中,72 h待其贴壁后,以丝裂霉素处理,与K562细胞共培养,计数法测定细胞增殖,观察成人骨髓MSCs对K562细胞生长的影响。结果:和单独的K562细胞生长曲线相比,与骨髓MSCs共孵育的K562细胞生长缓慢,无明显的指数生长期。结论:成人骨髓MSCs抑制K562细胞的生长,提示其可能有潜在的抗肿瘤作用,但其作用机制如何有待于进一步探讨。

Objective：To study the effects of adult human bone marrow mesenchymal stem cells（MSCs）on the growth of K562 cells in vitro. Method： Mononuclear cells were obtained from 5 ml adult human bone marrow by density gradient centrifugation with Percoll solution. Adult human MSCs were cultured in Dulbecco＇s Modified Eagle＇s Medium with low glucose （LG-DMEM） containing 10% fetal calf serum at a density of 2 × 10^5 cell/cm^2. The purity of MSCs were identified with the spindle-fibroblastic morphology by microphotograph and the phenotypes were analyzed by fluorescence-activated cell sorter （FACS）. The ultrastructure of MSCs was observed under transmission electron microscope. MSCs were plated in 24 well plates. After treated with mitomycin, MSCs were cocuhured with K562 cells. Effects of MSCs on the growth of K562 cells in vitro were investigated. The growth of K562 cells was measured by cell-count method. Result： Compared with the cell growth curve of the K562 cells alone, the K562 cells cocultured with MSCs grew slowly and the exponential phase of growth wasn＇t significant. Conclusion：Adult human bone marrow mesenchymal stem cells suppressed the growth of K562 cells in vitro. The results indicated that MSCs might have the potential to inbibit tumor cell growth. The mechanism was still to be investigated.