摘要
目的CD4+ CD25+Treg细胞介导的免疫调节对诱导和维持移植物免疫耐受有非常重要的作用,本研究探讨抗胸腺淋巴细胞球蛋白(ATG)和噻呢哌这两种不同作用途径的免疫抑制诱导药物对CD4+ CD25+high/CD4+T细胞及其表面标志性因子CTLA-4、Foxp3的影响,为临床合理选择应用免疫诱导药物提供依据。方法选择15例在我院行同种异体肝移植患者,随机分为ATG组(8例)和噻呢哌组(7例),两组除免疫抑制诱导药物不同外,均接受相同治疗。分别于术前、术后3周、6周、8周取血,应用流式细胞仪检测CD4+ CD25+high/CD4+ T细胞的数量及其表面因子CTLA-4和Foxp3表达状况,比较不同诱导药物对其影响。结果这两种免疫抑制诱导药物对CD4+ CD25+Treg细胞短期内有一定影响,但可逐渐恢复,两者比较,使用ATG后各指标恢复较噻呢哌快,尤其是Foxp3更加明显。结论作为诱导治疗药物,ATG更有利于CD4+ CD25+T细胞功能恢复,从而有助于更好形成移植免疫耐受。
Objective There is now a great degree of consensus that the CD4^＋ CD25^＋ regulatory T cells（ CD4^＋ CD25^＋ Treg ） play an important role in beth the induction and the maintenance of the transplant tolerance , so it is necessary to have intensive investigations on them, including the change of Treg cells and the expression of molecular markers , CTLA-4 and Foxp3. It is well known that the used immunosuppressive drugs currently may affect the number and the function of the CD4^＋ CD25^＋ Trig and as a result , hinder the establishment of transplant tolerance. This study focus on the impact of two inductive immunosuppressive drugs, anti-thymcyte globulin（ATG） and Zenapax on CD4^＋ CD2.5^＋ Treg in liver transplant recipients. Methods 15 liver transplant recipients aged 20 - 50 were chosen and divided into ATG group（ n = 8） and Zenapax group （ n = 7）. ATG or Zenapax were given as the inductive drugs. The maintenance drugs of the two groups are the same. FACScan Flow cytometry were used to test the CD4^＋ CD25^＋high/CD4^＋ ,CTLA-4 and Foxp3 of the blood sample at four time ends ： Pre-use, 3th week, 6th week and 8th week after transplantation. Results Though beth two inductive drugs can decrease the number of CD4^＋ CD25^＋ Treg and the expression of surface molecules, the Tregs can increase gradually. The ATG group recovered faster than the zenapax group. The difference was significant. Conclusion As the inductive drug, ATG may be better for the recovery of the CD4^＋ CD25^＋ Treg and as a result, better for the induction of transplant tolerance.
出处
《肝脏》
2007年第1期22-25,共4页
Chinese Hepatology
