牙及牙周组织联合切片的脱钙方法探讨

Study of methods of decalcification for making united slices of tooth and affiliated periodontic tissues

目的研究牙和牙周组织联合切片脱钙方法。方法成年犬磨牙及牙周组织标本21块,分成7组,分别用6种甲酸及 EDTA 脱钙,测定脱钙液的 pH 值、脱钙时间、标本重量、体积和脱出的钙量及各种染色效果等指标判定。结果脱钙液 pH 值低脱钙快,脱钙后标本重量减轻37.54%、体积缩小25.97%,每克湿重标本脱出的钙量为174.49 mg。EDTA 脱钙染色效果最佳,脱钙慢。Plank-Rvcho 脱钙液较迅速,切片质量和染色效果不佳。以氯化铝为保护剂的甲酸脱钙液,切片质量和染色效果优良,比 EDTA 脱钙迅速。结论以氯化铝为保护剂的50%甲酸是较理想的脱钙液。

Objective To study the methods of decalcification for making united slices of tooth and affiliated periodontic tissues. Methods Twenty-one samples containing dog molars and affiliated periodontic tissues were divided into seven mean groups. The pH value of solution, time of decalcification, weight and volume of samples, and content of decalcified calcium were detected. The slices were observed by HE, specific,and immunohistochemical stain. Results The velocity of decalcification increased with decrease of solution pH. The weight of samples lightened by 37. 61%, the volume reduced by 25.97% on average,and calcium decalcified was 174. 49rag per gram humid samples. The EDTA decalcification was slowest, but it was best. Decalcification was fast in Plank-Rycho solution while the section was worst, and faster in the formyl solution containing aluminium chloride than in EDTA, and the section was better. Conclusions The 50% formyl solution containing aluminium chloride is an ideal decalcifying solution.