摘要
目的探讨金黄色葡萄球菌肠毒素 B(staphylococcus aureus enterotoxin B,SEB)对原代培养的人鼻黏膜上皮细胞释放促炎因子和(或)趋化因子的作用。方法将无血清原代培养的人鼻息肉及下鼻甲上皮细胞分别在 SEB 1、10、100 ng/ml,白细胞介素(interleukin,IL)1β20ng/ml 及 SEB10 ng/ml+地塞米松13ng/ml 等不同条件下孵育,12、24和48h 后采用原位杂交方法检测鼻黏膜上皮细胞分泌的 IL-5和粒细胞巨噬细胞集落刺激因子(granulocyte macrophage colony stimulating factor,GM-CSF)在 mRNA 水平的变化。结果①SEB 刺激鼻黏膜上皮时,IL-5和 GM-CSF mRNA 表达增加,在一定范围内呈现时间和剂量依赖性,以10ng/ml、24h 时最明显(P<0.05),且鼻息肉组的表达高于下鼻甲组,差异具有统计学意义(P<0.05);②IL-1β 20n/ml 组 IL-5和 GM-CSF mRNA 表达增加不及 SEB 10ng/ml 组明显,差异具有统计学意义(P 值均<0.05);③SEB 10ng/ml+地塞米松13 ng/ml 组的 IL-5和 GM-CSF mRNA 表达强度较 SEB 10 ng/ml 组弱,差异具有统计学意义(P 值均<0.05)。结论 SEB 对原代培养的人鼻黏膜上皮细胞具有促炎作用。
Objective To evaluate the effects of staphylococcus aureus enterotoxin B (SEB) on proinflammatory cytokine/chemokine releases in primary human nasal epithelial ceil (HNEC). Methods Epithelial ceils of nasal polyps (NP) and inferior turbinate (IT) were cultured without serum under stimulus of SEB 1,10, 100 ng/ml, IL-1β 20 ng/ml and SEB 10 ng/ml + dexamethasone 13 ng/ml for 12,24 and 48 h, respectively. The expression of IL-5 and Granulocyte-macrophage colony stimulating factor(GM-CSF) mRNA derived from epithelial ceils was detected by in situ hybridization. Results ①The expression of IL-5 and GM-CSF mRNA was time and dose-dependent, and reached to a peak under SEB 10 ng/ml for 24 h (P 〈0. 05). The mRNA expressed more intensively in epithelial ceils from NP than IT( P 〈0. 05). ②The expression of IL-5 and GM-CSF mRNA increased less under the stimulus of IL-1β than SEB 10 ng/ml ( P 〈 0. 05 ). ③The mRNA level of IL-5 and GM-CSF decreased under the stimulus of SEB + dexamethasone 13 ng/m when compared with the stimulus of SEB 10 ng/ml ( P 〈 0. 05 ). Conclusion SEB showed proinflammatory effects on HNEC.
出处
《中华耳鼻咽喉头颈外科杂志》
CAS
CSCD
北大核心
2007年第3期202-206,共5页
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
基金
国家十五科技攻关基金(2004BA720A19-01)