南瓜多糖对四氧嘧啶致糖尿病小鼠降糖作用的研究

Study on Pumpkin Polysaccharide Reducing th Blood Glucose on the Diabetic Model Mouses by Alloxan

目的:观察南瓜多糖对Alloxan糖尿病小鼠的降糖作用。方法:实验选用雄性小鼠17g^22g,在日照时间10小时以上的阳光充足的条件下饲养。小鼠采用两次给药法腹腔注射四氧嘧啶,给药剂量分别为第1天为120mg/kg;第2天为100mg/kg,建立四氧嘧啶性糖尿病模型,用葡萄糖氧化本科法检测血糖值,用放射免疫分析法测定胰岛素、胰高血糖素,以观察各组的降糖效果。结果:南瓜多糖两种剂量组均可降低四氧嘧啶糖尿病小鼠的血糖(包括禁食空腹和进食以后)(P<0.05),且能明显提高血中胰岛素水平;减低血胰高血糖素浓度。结论:南瓜多糖对糖尿病小鼠有降糖作用。

Objective： To observe the function of pumpkin polysaccharide in reducing the blood glucose on the diabetic mode mouse by alloxan, Methods： On the condition of two-step treatment（120mg/kg in the first day and 100mg/kg in the second day）. Exposing to sunshine more than 10h in male mouse weighting 17g-22g. Alloxan was injectected into body of mouse to induce diabetic model. The blood glucose was detected by glucose oxidase. Blood insulate and glucagons was detected by radiommunoas-say. Results： The blood glucose and glucagons of model group treated with pumpkin polysaccharide was decreased by the two groups dosages（include fast and after fare）, insulate was increased. Conclusion： Pumpkin polysaccaride can reduce the blood glucose of diabetic mouses.