细胞因子Daintain/AIF-1的纯化及其单克隆抗体的制备与鉴定

Isolations and Identifications of Daintain/AIF-1 and the Monoclonal Antibodies against Daintain/AIF-1

由猪小肠中纯化鉴定Daintain/AIF-1,并以其为免疫原免疫BALB/c小鼠,用杂交瘤技术制备得到4株稳定分泌抗体的单克隆细胞株。对单抗效价、亚类、亲和常数进行分析,4株单抗都属于IgG1(κ)。又经West-ern blot证实,所制备的抗体能特异性与Daintain/AIF-1结合。

Daintain/AIF-1 is a recently discovered cytokine-like polypeptide. This bioactive peptide is involved in transplant rejection, autoimmune diseases and tumor generation and progress etc. Daintain/AIF-1 from porcine intestine was isolated and identified and used as antigen to manufacture monoclonal antibodies （Mabs）. Using hybridization technique, four cell clones producing Mabs against Daintain/AIF-1 were obtained. Isotypes, titer,affinity of the Mabs were also analysed. Those Mabs belong to G1 subclass with k light chain. The specificity of the Mabs was further verified by Western blot. In first time the monoclonal antibodies against Daintain/AIF-1 was prepared and identified, which will be useful tools for further investigation of transplant rejection,autoimmune diseases and cancer diagnosis.