阿维链霉菌aveC基因缺失对产素调控的影响

Effect of aveC Gene Deletion on Avermectins Production in Streptomyces avermitilis

通过构建仅含有aveC基因两端交换臂的缺失载体,与阿维链霉菌(Streptomyces avermitilis)染色体中aveC基因发生同源重组,以阻断该基因,从而获得aveC缺失突变株。并经摇瓶发酵和高效液相色谱检测不同阿维菌素组分产量。结果显示,aveC缺失突变株的阿维菌素的总产量与出发菌株的总产量差异不显著;而阿维菌素组分1的产量下降约58%,组分2的产量提高约52%。

Gene deletion vector aveC was used in Streptomyces avermitilis, aveC gene in the chromosome was displaced by deletion allele on the plasmid via double crossover. Disruptants were assued by PCR. Shaking flask experiments and HPLC analysis showed that the total production of avermectin in the deletion and the origin ones are at the same level. Compared with the origin Streptomyces avermitlis, the production of avermectin B1 in the mutants reduced about 58%, while that of avermectin 、B2 increased about 52%.