RP-HPLC法同时测定不同采收期连翘中乌索酸和齐墩果酸的含量

RP-HPLC determination of ursolic acid and oleanolic acid in various collecting period of Forsythia suspensa(Thunb.) Vahl.

目的建立同时测定连翘中乌索酸和齐墩果酸含量的测定方法。方法采用HPLC法测定其含量。色谱柱为NucleodurC18色谱柱（4．6mm×250．0mm，5μm），流动相为甲醇-水（体积比为88：12）。流速为0．8mL·min^-1，检测波长为210nm，柱温为25℃。结果乌索酸、齐墩果酸进样量分别在0．4265～4．2650μg（r=0．9997）和0．4624～4．6240μg（r=0．9999）内峰面积与进样量呈良好的线性关系；乌索酸和齐墩果酸的平均回收率分别为99．6％（RSD为0．7％）和98．7％（RSD为0．8％）。结论该方法可用于中药连翘的质量评价和控制。

Objective To develop a method for the quantitative analysis of ursolic acid and oleanolic acid in Forsythia suspensa simultaneously. Method HPLC was carried out on the Nucleodur C18 column （4.6 mm× 250.0 mm, 5 μm）at 25 ℃ using MeOH-H2O（ V： V = 88： 12）as mobile phase with a flow rate of 0.8μL. min^- 1 and detected at 210 nm. Results There was good liner relationship between the peak areas and the sample contents injected at the ranges of 0.426 5- 4.265 0 μg（ r = 0.999 7）and 0.462 4 4.624 0μg（ r = 0.999 9）for ursolic acid and oleanolic acid respectively. The average recovery rates of ursolic acid and oleanolic acid were 99.6 % （RSD 0.7 % ）and 98.7 % （RSD 0.8 % ）, respectively. Conclusion The method can be used for the quality evaluation and control of Forsythia suspensa.