低转移性和高转移性的FBJ骨肉瘤细胞在脂筏上的区别

Difference in lipid raft between lowly metastatic and highly metastatic FBJ cells

目的通过寻找低转移性和高转移性的FBJ骨肉瘤细胞在脂筏上的区别来研究神经节苷脂(GD1a)调控肿瘤转移能力的机制。方法提取细胞的脂筏成分,用聚丙烯酰胺凝胶电泳和二维电泳来检测转移性不同的细胞在脂筏上的蛋白组成区别,并用免疫印迹法(Western blot)来分析转移性不同的细胞在脂筏上的磷酸化区别。结果通过以上方法,发现了转移性不同的细胞在脂筏上的蛋白组成上有所不同,且磷酸化呈现明显区别,这些变化可能与调控肿瘤细胞的转移有关。

Objective to investigate the difference in lipid raft between highly metastatic and lowly metastatic FBJ cells and elucidate the regulating mechanism of ganglioside GDla on tumor metastasis. Methods After lipid raft fraction was extracted, the difference of protein composition in lipid raft between cells with different metastatic ability was analyzed by SDS-PAGE and two dimensional electrophoresis, and the difference in phosphorylation pattern between cells with different metastatic ability was analyzed by Western blotting. Conclusions Using the methods as above described, we find that there are some difference between highly metastatic cell and lowly metastatic cells both in protein composition and phosphorylation pattern, these changes maybe relate to the metastatic ability of tumor cells.