摘要
为了研究实验性变态反应性脑脊髓炎(EAE)的病理、组化的变化,通过对豚鼠EAE模型进行普通病理、电镜及单胺类介质荧光组化研究发现,动物发病前的组织虽在光镜下无明显病变,但电镜下可见髓鞘薄层环形结构松散、断裂或融合;薄髓鞘的轴索内可见线粒体肿胀和嵴消失,而厚髓鞘纤维轴索内的线粒体尚完好。病变以脊髓白质为主,脑的病变主要集中于脑室周围。病变以坏死为主,集中于小静脉周围,可见髓鞘脱失;炎细胞包绕小静脉呈袖套样改变。此外,EAE神经组织单胺类介质荧光密度显著减少,这可能是引起EAE动物肢体运动功能障碍的原因之一。
Thepathologicalchangesofexperimentalalergicencephalomyelitis(EAE)werestudiedintheguineapigswiththeaidofalightmicroscope(LM),anelectronmicroscope(EM)andfluorescenthistochemistry.NoevidentlesionswerefoundunderLMbeforetheclinicalonsetofEAE,butunderEMtherewasalotofloose,fusionalandfragmentalspiremesofmyelinsheath.Themitochondriaintheaxonsofthickmyelinsheathsappearedtobenormal,butthemitochondriainaxonsofthinmyelinsheathswereenlarged.Thebainlesionsweremainlylocatedinthewhitemat-ter,especialyaroundthecerebralventricle.Thedamagesinthespinalcordwereverysevere.Necrosiswasthemainpathologicchangeandusualyenclosedminorveins.Demyelinatingcouldbeseenandinflammatorycelsenclosingminorveinslikecufinginfiltration.Mostinflammatorycelswerelymphocytesandmacrophages.Otherwise,thefluorescentdensityofmonoaminesinEAEwassignificantlydecreased.Perhapsthisfindingmighthelpexplaintheimpairmentofthemovementoftheextremities.
出处
《中华神经科杂志》
CAS
CSCD
1996年第2期97-99,共3页
Chinese Journal of Neurology