白术黄芪汤及其有效部位组方对小鼠溃疡性结肠炎的疗效观察

Effects of Baizhu Huangqi Decoction and Its Effective-part Prescription on Mice Ulcerative Colitis

目的观察白术黄芪汤及其有效部位组方对小鼠溃疡性结肠炎模型的疗效。方法采用三硝基苯磺酸(TNBS)灌肠法对小鼠进行造模,以疾病活动指数、结肠黏膜组织损伤的大体和病理评分、髓过氧化物酶(MPO)活性为观察指标对其疗效进行综合评价。结果白术黄芪汤及其有效部位组方对造模小鼠的疾病活动指数、结肠黏膜损伤指数、病理组织学评分和MPO活性均有一定疗效;有效部位组方疗效略优,其与模型组比较差异均有显著性意义(P<0.01),与白术黄芪汤组比较差异也有显著性意义(P<0.05)。结论白术黄芪汤及其有效部位组方对小鼠溃疡性结肠炎均有一定疗效,以有效部位组方疗效较好。

Objective To observe the effects of Baizhu Huangqi Decoction and its effective -part prescription on mice ulcerative colitis （UC） . Methods The mice UC model was induced by clyster with 2, 4, 6 -trinitrobenzenesulfonic acid （TNBS） and the effects were comprehensively evaluated by disease activity index, the macroscopic and histological assessment of colon mucosa damage and the activity of myeloperoxidase （MPO） . Results Both Baizhu Huangqi Decoc- tion and its effective - part prescription had effects on disease activity index （DAI） , inflammation index and MPO activ- ity. The effect of effective - part prescription was better than that of UC model group （ P 〈 0.01 ） and Baizhu Huangqi Decoction group （ P 〈 0.05） . Conclusion Baizhu Huangqi Decoction and its effective - part prescription both have a certain effect on mice UC model, especially its effective - part prescription.