蟾毒它灵对人胃癌BGC-823细胞的促凋亡作用

Effect of Bufotalin on the Apoptosis of BGC-823 Cells

目的考察蟾蜍甾烯类化合物的生物转化产物蟾毒它灵(bufotalin)对BGC-823细胞的促凋亡作用,初步探讨其作用机理。方法MTT法检测细胞毒作用、Hoechst染色法和Annexin Ⅴ染色法检测细胞凋亡,免疫印迹杂交法检测裂解形式的caspase-3。结果蟾毒它灵在1μmol/L时对BGC-823细胞的毒性作用达到50%以上,形态学观察和流式细胞仪检测结果显示蟾毒它灵可明显促进BGC-823细胞的凋亡,上调caspase-3的表达。结论蟾毒它灵对BGC-823细胞有明显的促凋亡作用,其作用机理可能通过caspase-3凋亡途径实现。

Objective To investigate the effect of the bioconversion product of bufotalin from bufadienolide on the apoptosis of BGC - 823 cells and to explore its mechanism. Methods BGC - 823 cells were exposed to the compound and the cytotoxicity was measured with MTT assay. Hoechst 33258 staining and Annexin V staining were adopted to detect apoptosis, and western blotting method was used for the assay of cracked caspase - 3. Results Bufotalin showed a cytotoxicity against BGC - 823 cells in a concentration - dependent manner, the cytotoxicity of 1μmol was up to 50 % and above. Bufotalin promoted BGC - 823 cell apoptosis and increased the level of activated caspase - 3. Conclusion Bufotalin can induce the apoptosis of BGC - 823 cells and its mechanism may be through caspase - 3 pathway.