摘要
目的对猴枣牛黄散中的牛黄进行定性、定量的控制。方法①用薄层色谱法分别鉴别猪去氧胆酸和胆红素;②用薄层扫描法测定胆酸含量,扫描波长:λs=380nm,Rλ=650nm。结果薄层色谱法可以区分体外培育牛黄和人工牛黄。含人工牛黄的样品有猪去氧胆酸斑点,含体外培育牛黄的样品未检出有与猪去氧胆酸对照品相对应的斑点;含人工牛黄的样品不显胆红素斑点,含体外培育牛黄的样品显示与胆红素对照品相对应的斑点。薄层扫描法测定胆酸含量可以控制体外培育牛黄的质量,该法线性范围为0.2～1.8μg;平均回收率为99.5%,RSD为3.3%(n=6),测得结果符合药典关于体外培育牛黄标准的要求。结论所建立的方法能有效地控制猴枣牛黄散中牛黄的质量。
Objective To make a qualitative and a quantitative control on the Calculus Bovis in Houzao Niuhuang Powder. Methods Hyodeoxycholic acid and bilirubin in Calculus Bovis were separately identified by TLC, and the cholic acid content was determined by TLCS with the scan wavelength of λS = 380 nm, λR = 650 nm. Results TLC can be used for the identification in - vitro cultured Calculus Bovis and artificial Calulus Bovis, the samples containing cultured Calu- lus Bovis showed the specific spots of bilirubin but no hyodeoxycholic acid spot while the samples containing artifical Calulus Bovis showed the hyodeoxycholic acid spot but no bilirubin spot. TLC - scanning can be used to measure the quantity of cholic acid , with the linear range being 0.2 - 1.8 μg, average recovery rate of 99.5 % , and RSD being 3.3 % （ n = 6） , and the results accorded with the criterion of the cultured Calculus Bovis in Chinese pharmacopoeia. Conclusion The methods can effectively control the quality of Calculus Bovis in Hollzao Niuhuang Powder.
出处
《中药新药与临床药理》
CAS
CSCD
2007年第2期123-126,共4页
Traditional Chinese Drug Research and Clinical Pharmacology
