富硒麦芽对二乙基亚硝胺致肝癌大鼠低血糖症及相关体液调节因子动态变化的影响

Effect of Selenium-enriched Malt on Dynamic Variation of Hypoglycemia and Relative Humoral Regulation of Hepatocarcinoma Rats Diethylnitrosamine-induced

雄性SD大鼠193只,体重100-120 g,随机分成5组。组Ⅰ-组Ⅲ,日粮中添加富硒麦芽(SEM)至硒含量分别为0.3、1.0、3.0 mg/kg;组Ⅳ,模型组;组Ⅴ,阴性对照组,不加SEM也不诱癌,日粮中分别添加亚硒酸钠至硒含量达0.1 mg/kg。组Ⅰ-组Ⅳ,饮水中添加100 mg/L二乙基亚硝胺(Diethylnitrosamine,DEN)诱癌,维持DEN摄入量每天约10 mg/kg体重,连续16周,然后改饮普通灭菌水至18周末。组V始终以普通灭菌水自由饮用。每4周眼眶采血1次,测定血浆葡萄糖及其相关体液调节因子胰岛素、胰高血糖素、胰岛素样生长因子-Ⅱ(IGF-Ⅱ)等的含量,统计胰岛素/葡萄糖(IGR1)、胰岛素/胰高血糖素(IGR2)、胰高血糖素/葡萄糖(GGR)值。分析血糖与各相关调节因子间的相关性。结果发现,与同期阴性对照组相比,各诱癌组大鼠血糖和血清胰岛素、胰高血糖素水平及GGR值均显著下降,血清IGF-Ⅱ水平和IGR2值则显著上升,而某些剂量的SEM能有效地抑制血糖、胰高血糖素水平的下降和血清IGF-Ⅱ水平I、GR2值的上升。血糖与上述各调节因子间分别存在显著相关性。结果表明SEM能抑制肝癌大鼠低血糖症的发生,该作用可能是通过对诸多相关调节因子的共同调节而实现的。

193 SD rats weighting 100-120 g were divided randomly into 5 groups. The animals in Group Ⅰ , Ⅱ and Ⅲ were fed with selenium as Se-enriched malt（SEM）supplemented diets （0.3, 1.0 and 3.0 mg/kg）. Animals in group IV and group V received 0.1 mg/kg selenium in sodium selenite only and were severed as the positive control and negative control respectively. Animals of groups Ⅰ -Ⅳ were induced hepatoma by diethylnitrosamine resolved with sterilized water （100 mg/L） at the dosage of 10 mg/kg body weight every day for 16 weeks,then drank with sterilized water for another 2 weeks. Eyeground blood samples of five animals selected randomly from each group were respectively collected after 12 hours＇ fast in every 4 weeks. Then the values of plasma glucose at different sampling time were measured. Subsequently, the values of the hormones related to plasma glucose metabolism, including insulin, glucagon, and the ratios of insulin/glucose （IGR1）, insulin/glucagon （IGR2）, glucagon/glucose （GGR） and insulin-like growth factors-Ⅱ （IGF-Ⅱ） were determined. At the same time, the correlation of the plasma glucose concentrations related to hormones was analyzed respectively. The results indicated that the values of plasma glucose and the relative hormones, including insulin, glucagon and GGR in the groups treated with DEN were decreased significantly compared with the negative control group, however, the values of IGF-Ⅱ and IGR2 were increased significantly. SEM showed a significant effect in suppressing the decreasing of plasma glucose and glucagons, delaying the increasing of IGF-Ⅱ and IGR2 in the DEN-induced hepatoma rats. The plasma glucose concentrations revealed a significant relation to the hormones. In conclusion, SEM can efficiently deaden the development of hypoglycemia in the DEN-induced hepatoma rats by regulating the mutifactors metioned, including the levels of the relative hormones and balance among the hormones.