不同强度康复训练对脑缺血再灌注大鼠运动功能和胶质纤维酸性蛋白表达的影响

The effects of rehabilitation intensity on motor function and GFAP expression in rats after cerebral ischemiareperfusion

目的探讨应用不同强度康复训练对脑缺血再灌注大鼠运动功能以及海马区和梗死灶周围胶质纤维酸性蛋白（GFAP）表达的影响。方法采用大鼠局灶性脑缺血再灌注模型，大脑中动脉阻塞1h，再灌注7d和14-d，36只造模成功的大鼠随机分为造模对照组、常规康复训练组和强化康复训练组，分别采用姿势反射试验、肢体不对称应用试验和角落试验观察各组大鼠的运动功能，应用免疫组织化学方法分别检测各组大鼠缺血侧海马区和梗死灶周围GFAP的表达情况。结果缺血再灌注7d和14d时，两个康复训练组大鼠行为学评分优于造模对照组，其GFAP表达的光密度值高于造模对照组；缺血再灌注14d时，强化康复训练组大鼠行为学评分优于常规康复训练组，其GFAP表达的光密度值高于常规康复训练组。结论康复训练可促进脑缺血再灌注大鼠运动功能的恢复和星形胶质细胞的活化，强化康复训练的效果更明显。

Objective To explore the effects of rehabilitation training （RT） at different intensities on motor function （MF） and glial fibrillary acidic protein （GFAP） expression after cerebral ischemia and reperfusion （CIR）. Methods The middle cerebral arteries （MCA） of 36 rats were occluded for 1 h and then reperfused for 7 or 14 days. They were then divided randomly into a control group, a conventional rehabilitation group （CR group） and an inten- sive rehabilitation group （IR group）. Their motor function was measured by means of a postural reflex test, an asym- metrical limb use test and a corner test, and the expression of GFAP in the hippocampus and the peri-cerebral infracted area was detected using immunohistochemistry. Results Compared with those in the control group, the neurological function scores and the GFAP expression in the CR and IR groups were significantly increased at both day 7 and day 14 after CIR, while these parameters in the IR group were increased in comparison with those in the CR group at day 14. Conclusion RT, especially intensive rehabilitation, can improve the recovery of motor function and activate astrocytes in rats after CIR.