针刺对缺血性脑卒中大鼠血清髓鞘碱性蛋白含量及缺血灶神经髓鞘再生的影响

The effects of acupuncture on serum myelin basic protein and remyelination after ischemic stroke

目的观察针刺对缺血性脑卒中大鼠受损脑髓鞘的保护作用并探讨其相关机制。方法共选取100只成年雄性SD大鼠，将其随机分为正常组、模型组、早期针刺组（早期组）及晚期针刺组（晚期组）。采用改良的线栓法制备右侧大脑中动脉阻塞（MCAO）的局灶性脑缺血再灌注模型。分别于造模后不同时间点采用“醒脑开窍针刺”法对早期组及晚期组大鼠进行针刺干预。采用Pal-Weigert髓鞘特殊染色法和ELISA法动态观察各组大鼠在实验开始1，3，5，7d时脑髓鞘的再生情况及血清髓鞘碱性蛋白（MBP）含量的改变。结果在各不同观察时间点，发现模型组大鼠内囊髓鞘均有明显脱失，且血清MBP含量亦较造模前明显升高；各针刺组大鼠在相应时间点的髓鞘脱失程度均较模型组明显减轻，且血清MBP含量升高的幅度亦显著低于模型组；另外早期组大鼠血清MBP含量达到峰值的时间较晚期组滞后。结论针刺可能通过激发大鼠脑可塑性潜能从而促进MCAO脑缺血损伤区的神经髓鞘组织再生，并且针刺治疗时间窗对缺血后脑损伤的恢复具有重要影响作用。

Objective To study the protective effects of acupuncture on injured cerebral myelin in rats with focal cerebral isehemia. Methods One hundred adult male Sprague Dawley rats were randomly divided into a normal group （N）, an MCAO group （C.）, an early acupuncture group （E2） and a late acupuncture group （E3 ）. Focal cerebral ischemia and reperfusion models were established by middle cerebral artery occlusion （MGAO） on the right side using a modified thread embolism method. Xingnao kaiqiao acupuncture needling was applied for treatment. Serum myelin basic protein （MBP） and remyelination of the brain in all groups was observed at 1,3,5 and 7 days by using Pal Weigert myelin staining and EL1SA methods. Results Massive demyelination in the internal capsule was observed in the C. group, and serum MBP was markedly increased. In the E2 and E3 groups, the demyelination was distinctly less, and serum MBP was much lower than in the C . group. Serum MBP levels peaked in the E2 group later than in the E3 group. Conclusion Acupuncture can promote remyelination of the ischemic region after MCAO in rats. It may promote plasticity. The duration of acupuncture treatment may play an important role during recovery after cerebral ischemia.