N末端脑钠肽的定量ELISA法测定与临床应用

Enzyme-Linked Immunosorbent Assay for the Quantitative Determination of N-Terminal Pro-Brain Natriuretic Peptide in Serum and Its Clinical Application

目的建立N末端脑钠肽（NT—proBNP）的定量ELISA法测定，并探讨其应用价值。方法用定量ELISA法测定患者和健康组的血清NT—proBNP。结果该法线性为0．6～1200pmol／L，平均回收率为98．5％，批内CV为4．36％，批间CV为6．16％。29例心绞痛和21例风湿性心脏病及23例急性心肌梗死患者的1gNT—proBNP值分别为（2．83±0．21）pmol／L和（3．03±0．20）pmol／L及~（3．21±0．22）pmol／L。结论该法灵敏、特异和准确，血清NT—proBNP定量检测是评价心功能的最佳实验指标。

Objective To estab-lish enzymelinked immunosorbent assay（ELISA） for the quantitative determination of N- terminal pro-brain natriuretic peptide（NT-proBNP） in serum and to investigate its clinical application. Methods Serum NT-proBNP concentrations of patients and of normal controls were measured by ELISA. Results The linearity was 0. 6- 1 200 pmol/L, The average rate of recovery was 98.5%. Within-run CV was 4.36% and between-run CV was 6.16%. The serum Log NT-proBNP concentrations of 29 patients with angina pectoris were （2.83 ±0. 21）pmol/L,of 21 patients with rheumatism of the heart were（3.03±0. 20）pmol/L and of 23 patients with acute myocardial infarction were（3.21±0. 22） pmol/L. Conclusion This method is sensitive,specific and accurate. Quantitative detection of NT-proBNP is now the best laboratory test for evaluating heart function of patients.