盐度对凡纳滨对虾幼虾消化酶活性的影响

Effect of salinities on digestive enzyme activities of juvenile Litopenaeus vannamei Boone

采用酶学分析方法,在盐度为1,15和30的条件下,对凡纳滨对虾(Litopenaeus vannamei Boone)幼虾消化酶活性进行测定。实验结果表明:(1)幼虾肝胰腺中,当盐度为1时,胃蛋白酶活性最高[29.58μg/(mg..min)],胰蛋白酶活性[92.46μg/(mg.min)]与盐度为15时的值[97.60μg/(mg.min)]相当;胃肠中,胰蛋白酶和胃蛋白酶活性均以盐度为1时最高。(2)当盐度为1时,肝胰腺和胃肠中脂肪酶活性最大,但统计分析各组间差异不显著(P>0.05)。(3)肝胰腺中,各盐度组的淀粉酶活性很接近,组间无显著差异(P>0.05);胃肠中,盐度为1时的淀粉酶活性最高,达109.29μg/(mg.min),显著高于盐度为15,30时的活性(P<0.05)。

In this paper, the effect of salinity on digestive enzyme activities of the juvenile Litopenaeus vannamei Boone was studied by means of enzyme analysis. The experiment was made under three levels of salinities equal to 1, 15and 30 respectively. The results were as follows： （1）In the hepatopancreas of juvenile Litopenaeus vanname Boone under the salinity equal to 1, the pepsin has the highest activity[29.58μg/（mg·min）], and the activity of tyrsic-like[92.46μg/（mg·min）] corresponds to the highest[97. 60μg/（mg·min）] under the salinity equal to 15, and in stomach, both tyrosic-like and pepsin have the highest activity. （2）The lipase has the highest activity in hepatopancreas and stomach under the salinity equal to 1, but the differences among various groups are not prominent （P〉0.05） through the statistical analysis.（3）The activities of amylase are equite contiguous in hepatopancreas, and there is no prominent difference among various groups of salinities（P〉0.05）, and in stomach the juvenile Litopenaeus vannamei Boone＇s amylase has the highest activity under the salinity equal to 1, and reaches 109.29 μg/ （mg·min） ,prominently higher than that under the groups of salinities equal to 15 and 30 （ P〈0.05 ） .