羽苔素E体外抗真菌活性及逆转氟康唑耐药的初步研究

Primary Study on Antifungal Activities and Reversal of Fluconazole Resistance of Plagiochin E

目的评价白色念珠菌对羽苔素E的体外敏感性,并观察羽苔素E和氟康唑合用对白念敏感株和耐药株的联合抗真菌作用。方法采用微量稀释法,测定15株白念敏感株和4株白念耐药株对羽苔素E的敏感性,以最低抑菌浓度(MIC)表示。同时观察羽苔素E与氟康唑合用对白念敏感株和耐药株的联合抗真菌作用。结果羽苔素E对敏感株的MIC值为16 mg.L-1,对耐药株的MIC值与敏感株基本一致。羽苔素E与氟康唑合用,对敏感株和耐药株分别表现出相加和协同的抗真菌活性,并明显减少氟康唑的用量。结论羽苔素E具有中等的抗真菌活性,与氟康唑合用对耐药菌株表现出协同的抗真菌作用,提示羽苔素E有较好的抗真菌作用并有逆转氟康唑耐药的潜力。

OBJECTIVE To investigate the antifungal activity of plagiochin E against the strains of Candida albicans in vitro, and the interactions of fluconazole with plagiochin E against the sensitive and resistant strains. METHODS A broth micro-dilution method was used to determine the MICs of plagiochin E for the fifteen sensitive and four resistant strains. The combination effect of plagiochin E and fluconazole was also assessed. RESULTS The MIC of plagiochin E was 16 mg · L^-1 against the fifteen sensitive strains. Plagiochin E had the same MIC against resistant strains. After the administration of plagiochin E and fluconazole at the same time, MICs of two drugs were lower against both resistant strains and sensitive ones. Additive effect on sensitive strains and synergic effect on resistant strains were observed respectively. CONCLUSION Plagiochin E has a moderate but significant antifungal action on the sensitive and resistant strains of C. albicans, and shows additive or synergy effect after the administration of fluconazole. Plagiochin E can be useful for antifungal therapy and has a potential action on reversing the resistance of C. albicans against flueonazole.