MAGE-A1在食管癌中的表达及其与肿瘤细胞增殖的关系

Expression of melanoma antigen A1 and its relationship with tumor cell proliferation in esophageal carcinoma

目的:研究食管癌中肿瘤特异性抗原MAGE-A1,Ki-67的表达及其相关性,探讨他们与食管癌生物学行为及生存时间的关系.方法:免疫组化SP法,利用鼠抗人MAGE-A1,Ki-67单克隆抗体检测60例食管癌标本中MAGE-A1,Ki-67表达水平.结果:60例食管癌中MAGE-A1表达率为83.3%(50/60),高表达率为10%(6/60),低表达率为73.3%(44/60),正常组织表达率为0%,食管癌和正常组织之间的表达具有显著性差异(P<0.001).Ki-67表达率为93.3%(56/60),高表达率为70%(42/60),低表达率为23.3%(14/60),正常组织表达率为13.3%(4/30),食管癌和正常组织之间的表达具有显著性差异(P<0.001).MAGE-A1与分化程度有关(P<0.05),Ki-67与年龄、分化程度、病理类型有关(P<0.05).MAGE-A1阳性表达的高低与Ki-67阳性表达的高低呈负相关(r=-2.91,P<0.05).MAGE-A1阳性患者的生存时间明显高于阴性者.结论:MAGE-A1在食管癌中有较高的表达,并且与细胞增殖相关,正常组织不表达,MAGE-A1阳性患者的预后好,有望作为免疫治疗的靶点.

AIM： To investigate the expression of melanoma antigen A1 （MAGE-A1） and Ki-67 in esophageal cancer （EC） and their correlations with the clinical pathological features and survival time of EC patients. METHODS： The expression of MAGE-A1 and Ki-67 was detected by SP immunohistochemical technique using mouse anti-human MAGE-A1 and Ki-67 antibodies in formalin-fixed and paraffin-embedded sections from 60 EC patients. RESULTS： The positive rate of MAGE-A1 expression was 83.3% （50/60） in EC, with a high expression rate of 10% （6/60） and low expression rate of 73.3% （44/60）, while the positive rate of MAGE-A1 expression was 0% in the normal esophageal tissues, significantly lower than that in EC tissues （P 〈 0.001）. The positive rate of Ki-67 expression was 93.3% （56/60） in EC, with a high expression rate of 70% （42/60） and low expression rate of 23.3% （14/60）, while the positive rate of Ki-67 expression was 13.3% （4/30） in esophageal normal tissues, significantly lower than that in EC tissues （P 〈 0.001）. There was a negative correlations between the positive expression of MAGE-A1 and Ki-67 （r = -2.91, P 〈 0.05）. Besides, MAGE-A1 expression was also correlated with the differentiation degrees of carcinoma （P 〈 0.05）, and Ki-67 expression was correlated with the age of patients, differentiation degrees and pathological type of carcinoma （P 〈 0.05）. The survival time was higher for patients with the positive expression of MAGE-A1 than that for patients with the negative MAGE-A1 expression （P 〈 0.05）. CONCLUSION： MAGE-A1 is highly expressed in EC patients, which is correlated with the proliferation of tumor cells. The patients with positive MAGE-A1 expression have a favorable prognosis.