热休克蛋白70对应激性胃溃疡黏膜的保护作用

Protective role of heat shock protein 70 for gastric mucosa with stress ulcer

目的:探讨热休克蛋白70(HSP70)表达是否随应激性胃溃疡病程的进展而变化.方法:收集30例应激性胃溃疡出血患者及10例术前患者的胃液及外周血,采用TRIzol一步法提取胃液脱落细胞及外周血白细胞总RNA,采用半定量RT-PCR检测HSP70的表达;同时,采用免疫组化技术检测外周血白细胞HSP70的表达.结果:RT-PCR检测结果提示应激性溃疡各期的HSP70表达明显高于对照组的表达(血标本:1期0.6289±0.1839,2期1.1322±0.3683,3期0.6855±0.1923,对照组0.3868±0.2071;胃液标本:1期0.7741±0.2442,2期1.2385±0.4558,3期0.8790±0.2722,对照组0.4626±0.2416;均P<0.05),应激性溃疡2期HSP70表达显著高于1期和3期(P<0.05),而1期和3期之间无统计学差异(P>0.05).免疫组化结果为应激性溃疡患者外周血白细胞HSP70的表达水平在不同病期有所不同(1期40%,2期80%,3期30%,对照组10%,χ2=10.8383,P<0.05),以2期表达水平最高,对照组表达最低.结论:应激性溃疡时患者外周血细胞和胃液脱落细胞均可检测到HSP70的表达,应激状态可诱导HSP70的表达,HSP70对胃黏膜具有保护作用,并可能促进应激性溃疡的愈合.

AIM： To investigate whether the expressive intensity of heat shock protein 70 （HSP70） is changed with the progression of stress ulcer. METHODS： Thirty stress ulcer patients with bleeding and ten patients without bleeding before operation from intensive care unit were included as study subjects. Gastric fluid and blood specimen were collected from these patients. After total RNA was extracted from the shed cells in gastric juice and peripheral white blood cells （PWBC） by TRIzol method, semi-quantitative reverse transcription-polymerase chain reaction （RT-PCR） was used to investigate the intensity of HSP70 expression. Meanwhile, immunohistochemistry was used to evaluate the expression of HSP70 in PWBC. RESULTS： RT-PCR result showed that the expressive intensity of HSP70 in each stage of stress ulcer was remarkably higher than that in the control group （blood specimen： 0.6289 ±0.1839, 1.1322 ± 0.3683, 0.6855 ± 0.1923 vs 0.3868 ± 0.2071; gastric fluid specimen： 0.7741 ± 0.2442, 1.2385 ± 0.4558, 0.8790 ± 0.2722 vs 0.4626 ± 0.2416; for stage 1, 2, 3 and control group, respectively; all P 〈 0.05）. The expression of HSP70 in stage-2 ulcer was higher than that in stage-1 and -3 one （P 〈 0.05）, but there was no significant difference in the expressive intensity of HSP70 between stage-1 and -3 ulcer （P 〉 0.05）. Immunohistochemistry showed that the level of HSP70 expressive was significantly different among stress ulcer of different stages and the control group （40%, 80%, 30%, 10%, for stage 1, 2, 3 and control group, respectively, x^2=10.8383, P 〈 0.05）, and the highest expression level occurred in stage-2 ulcer and the lowest in the control group. CONCLUSION： HSP70 expression can be detected both in the shed cells in gastric fluid and PWBC from patients with stress ulcer. HSP70 may protect gastric mucosa from stress-induced damage and promote the healing of ulcer.