健脾疏肝方对反流性食管炎大鼠模型食管组织病理及PCNA的影响

Effect of liver-soothing and spleen-strengthening decoction on esophageal pathology and proliferating cell nuclear antigen expression in rats with reflux esophagitis

目的:观察健脾疏肝方对反流性食管炎大鼠模型食管组织病理及增殖细胞核抗原(PCNA)表达的影响.方法:采用“不全幽门结扎+贲门肌切开术”制作动物模型,240只大鼠分为正常组、模型组、安慰剂(谷维素)组、阳性对照组(加斯清)、治疗高、中、低剂量组.给予相应处理后,取食管组织行HE染色,光镜下观察组织病理学变化,用免疫组化的方法测PCNA表达.结果:健脾疏肝方能显著改变反流性食管炎的病理变化,健脾疏肝方高剂量组食道组织病理变化明显减轻;健脾疏肝方能显著抑制PCNA表达的升高,与模型组相比PCNA的表达显著降低(小剂量:284.83±30.49vs330.00±35.35,P<0.05;中剂量:239.67±28.84vs330.00±35.35,P<0.01;高剂量:203.00±25.29vs330.00±35.35,P<0.01),其疗效与加斯清相当.结论:健脾疏肝方有助于降低PCNA的高表达,并有抗胃食管反流,减轻食管炎症的疗效.

AIM： To investigate the effect of liver-soothing and spleen-strengthening （LSSS） decoction on esophageal pathology and proliferating cell nuclear antigen （PCNA） expression in rats with reflux esophagitis. METHODS： Partial pylori ligation plus cardiomyotomy was used to establish the rat models of reflux esophagitis. A total of 240 rats were divided into 7 groups, including normal group, model group, placebo group, positive control group, and Chinese medicine groups （high-, moderate-, and low-dose group）. After treatment with the corresponding methods, HE staining was performed to observe the pathological changes of esophageal tissues, while the expression of PCNA was measured by immunohistochemistry. RESULTS： LSSS decoction remarkably alleviated the esophageal pathological changes, the pathological score was significantly different between high-dose LSSS decoction group and model group. As compared with that in model group, the expression of PCNA was significantly decreased in LSSS decoction groups （low dose： 284.83 ± 30.49 vs 330.00 ± 35.35, P 〈 0.05; moderate dose： 239.67 ± 28.84 vs 330.00 ± 35.35, P 〈 0.01; high dose： 203.00 ± 25.29 vs 330.00 ± 35.35, P 〈 0.01）. There was no marked difference between LSSS decoction groups and positive control group. CONCLUSION： LSSS decoction may restrain the high expression of PCNA while alleviate the symptoms and esophageal inflammation of reflux esophagitis.