银屑病患者皮损中类浆细胞树突状细胞和IRF-7,IFN-α mRNA表达研究

Expression of Plasmacytoid Dendritic Cells,IRF-7,IFN-α mRNA in the Lesions of Psoriasis Vulgaris

目的探讨银屑病患者皮损中类浆细胞树突状细胞(plasmacytoid dendritic cells,PDCs)和干扰素调节因子7(IRF-7)、干扰素α(IFN-α)mRNA的表达及其意义。方法采用免疫组织化学和逆转录-聚合酶链反应(RT-PCR)方法检测了22例银屑病患者皮损中PDCs和IRF-7,IFN-α mRNA的表达。15例整形外科患者的皮肤作为正常人对照。结果免疫组化结果显示银屑病组皮损中CD123阳性的PDCs表达明显增多,而在正常对照组没有表达;RT-PCR检测显示,与正常人皮肤相比,寻常性银屑病患者皮损中IFN-α mRNA的表达无实质性上调,差异无显著性,但是IRF-7 mRNA的表达水平明显增高,差异有显著性(P<0.001)。结论银屑病患者皮损中PDCs浸润,IRF-7 mRNA的表达水平上调可能是银屑病发生和发展的原因之一。

Objective To investigate the expression of plasmacytoid dendritic cells （PDCs）, interferon regulate factor-7 （IRF-7） and interferonalpha （ IFN-α ） mRNA in skin lesions of patients with psoriasis vulgaris. Methods The expressions of PDCs,IRF-7 ,IFN-α mRNA in the lesional skin of psoriasis vulgaris were detected by immunohistochemical technique （SP） and RT-PCR. As control,normal skin of healthy volunteers was also tested. Results The immunohistochemistry study showed that the expression of PDCs in the psoriatic lesions was significantly higher than that in the normal controls. According to the results of RTPCR,the mRNA expression of IRF-7 were greatly higher than that in normal controls,but no difference for IFN-α. Conclusion These findings indicate that up-regulated expression of PDCs, IRF-7 mRNA might be related to the pathogenesis of psoriasis.