补阳还五汤水煎剂中黄芪甲苷 阿魏酸 多糖的含量测定

Test the Content of Astragaloside Ferulic Acid and Polysaccharide in Buyang Huanwu Decoction

目的:研究补阳还五汤水煎剂中黄芪甲苷、阿魏酸、多糖的含量测定,并作为该方的质量控制标准。方法:黄芪甲苷采用高效液相色谱—蒸发光散射检测,阿魏酸采用高效液相色谱法,多糖采用苯酚—硫酸法。结果:黄芪甲苷在0.24-6.00μg与峰面积积分值呈良好线性关系(r=0.9999),平均回收率100.79%。阿魏酸在0.08-0.24ug与峰面积积分值呈良好线性关系(r=0.9999),平均回收率101.76%。多糖测得的平均回收率为102.80%。结论:黄芪甲苷、阿魏酸的含量测定,可作为补阳还五汤的质量控制标准。

Objective ： Study the method of testing astragaloside, ferulic acid and polysaccharide in Buyang Huanwu Decoction,take it as the Quality Standard of Buyang Huanwu decoction. Methods： HPLC -ELSD was used to assay the contents of astragaloside and HPLC was used to assay femlic acid,phenol- sulphuric acid was applied to assay Polysaccharide. Results： The good linear correlation of astragaloside was observed from 1.08μg to 12.96μg（ r =0. 9995） and that of femlic acid was observed from 0.4μg to 4.0μg （ r = 0. 9995 ）. average rate of recovery of polysaccharide was 102.80%. Conclusion： the contents of astragaloside and femlic acid may take as quality standard ofBuyang Huanwu decoction.