HPLC法测定芍药甘草汤总有效部位中4个活性成分的含量

Determination of Paeoniflorin Liquiritin Liquiritigenin and Ammonium Glycyrrhizinate in Total Effective Fractions from Paeornia and Glycyrrhiza Decoction

目的:建立芍药甘草汤总有效部位(TPG)中芍药苷、甘草苷、甘草素、甘草酸单铵盐的含量测定方法。方法:通过高效液相色谱法(HPLC),测定TPG中4种有效成分的含量。色谱柱:LichrospherC18(250mm×4.6mm,5μm);流动相A为乙腈,B为0.1%磷酸,作梯度洗脱。0、18、30、40、50、55、60、65min时,A(%)为14、20、28、35、40、60、14、14;流速:1mL.min-1;测定波长:230nm;柱温:25℃。结果:测得TPG中芍药苷、甘草苷、甘草素、甘草酸单铵盐的含量。结论:本方法简便、快捷、重现性好,可用于同时测定TPG中4种有效成分的含量,全面控制TPG的质量,确保每批产品的均一性。

Objective： To establish a sensitive and specific HPLC method for controlling the quality of total effective fractions from Paeornla and Glyeyrrhiza Decoction （TPG）. Methods： We used HPLC to determinate the contents of Paeoniflorin, Liquiritin,Liquifitigenin and Ammonium glycyrrhizinate in TPG, HPLC analysis was performed on a Lichrospher - C18 （250mm ×4.6mm,5μm ） ,with the mixture of acetonitrile （A） and 0.1% phosphoric acid - water （B） as mobile phase in gradient mode. The concentrations of solvent A were 14% ,20% ,28% ,35% ,40% ,60%, 14% and 14% at 0,18,30,40,50,55,60 and 65min , respectively. The column temperature was set up at 25℃ and the flow rate wasl mL - min^-1. Results ： We received the contents of the four effective fractions respectively. Conclusion ： This method is simple, quick and reproducible and could be used for the determination of the four constituents in TPG.