琴鱼的核型、C带和银染分析

The Karyotypes,C-bands and Ag-NORs of Ctenogobius giurinus

采用秋水仙素-低渗-空气干燥法和银染，以肾细胞为材料，对琴鱼（Ctenogobius giurinus）染色体的核型、C带和银染（Ag-NORs）进行了研究。结果表明：（1）琴鱼2n=44，核型组成为2n=8m＋12sm＋24t，NF=64，没有异型性染色体分化；（2）所有染色体着丝粒为阳性C带，而且第1对染色体短臂也呈C带阳性，其着色强度与该对染色体上的着丝粒C带相同；（3）Ag-NORs数目在不同细胞中表现出多态性，数目为1～2个，出现1个Ag-NORs的频率最低（9．8％），出现2个Ag-NORs的频率最高（78％），Ag-NORs主要出现在m1对和sm3对同源染色体上。

The chromosome specimens of Ctenogobius giurinus were obtained from metaphase of kidney cells by vivo-injection of PHA and Colchicine, lower osmotic pressure process, hypatoic-air drying technique, and then their karyotypes, C-bands and Ag-NORs were studied in this paper. The results showed- （ 1 ） Ctenogobius giurinus had a diploid chromosome number of 44 and its karyotype formula was 8m ＋ 12sm ＋ 24t, NF = 64, sex chromosome was not found; （2）All the centromeres of chromosomes were C-bands positive and so was the short arm of the 1^th chromosome with tinctorial strength same as its centromeric C-bands; （3） The Ag-NORs polymorphisms were individually specific, mainly 1 -2 pairs of the number, and the frequency of 1 Ag-NORs was the lowest（9.8% ） , while the frequency of 2 Ag-NORs was the highest （78%）, Ag-NORs mostly appeared in chromosome m1 and sm3.