摘要
以H5N1禽流感病毒株Ck／HK／Yu22／02作为抗原，应用常规杂交瘤技术和血凝抑制实验筛选出抗H5亚型禽流感病毒血凝素蛋白的单抗8H5，单抗8H5经免疫荧光鉴定具有很好的H5特异性。选择33株2002～2006年不同地域，不同宿主中分离的不同遗传变异亚系的H5N1病毒代表株，对单抗8H5分别进行血凝抑制实验及中和试验分析，结果显示单抗8H5对所有H5亚型病毒均有较强反应，而对非H5亚型标准病毒株均不反应，说明8H5是一株广谱性抗H5特异性中和单抗，并提示单抗8H5的HA识别表位可能是一个相当保守的中和表位。并且单抗8H5双抗夹心系统的初步评价显示了其在诊断应用上的前景。
The monoclonal antibody （McAb） 8H5 against the haemagglutinin of the H5 subtype avian influenza virus （AIV） was developed by fusing SP2/0 myeloma cells with the spleen cells of BALB/c mice immunized with Ck/HK/Yu22/O2（HSN1）. McAb 8H5 was nonreactive to HS-HA by indirect immune fluorescence test and hemagglutinin inhibition test （HI） with 13 strains of non-H5 standard AIV. McAb 8H5 showed high reacitivity with 33 strains of HSN1 virus isolated from 2002 to 2006 in different areas and hosts by HI test and neutralization test （NT）. These results suggested that McAb 81-15 is a special broadspectrum neutralization monoclonal antibody against haemagglutinin of H5 subtype AIV and recognizes a very conserved epitope in HA. Based on McAb 8H5, a diagnostic assay for HSN1 viruses was developed and the results suggested that McAb 8H5 will be of great use in diagnosing HSN1 viruses.
出处
《病毒学报》
CAS
CSCD
北大核心
2007年第2期85-90,共6页
Chinese Journal of Virology
基金
国家禽流感防治专项(2004BA519A73)
福建省科技重点项目(2005Y020)
福建省科技重大专项(2004YZ01-1)
教育部跨世纪优秀人才培养计划。
