摘要
The nucleotide sequence of shallot yellow stripe virus from onion(SYSV-O)in Shaanxi Province of China was described.It was 10427 nucleotides(nt)in length excluding the poly(A)tail and was predicted to encode a polyprotein of 3340 amino acids(aa)with a calculated molecular weight of 379.7kD.SYSV was most closely related to onion yellow dwarf virus(OYDV)and resembled it in having a much larger P3 protein than other species in the genus.SYSV-O shared 93.2% nt identity to the published sequence of a welsh onion isolate of SYSV(SYSV-ZQ2).The two isolates differed in NIb-CP cleavage site,that of SYSV-O was Q/V but Q/A in SYSV-ZQ2.Nucleotide identity of coat protein genes amongst all SYSV sequences ranged from 87.3%-84.1%.Phylogenetic tree analysis based on the 3’-terminal nucleotide sequences of SYSV isolates confirmed the existence of four distinct groups that were partially related to geographical origin and host plant species.
The nucleotide sequence of shallot yellow stripe virus from onion （SYSV-O） in Shaanxi Province of China was described. It was 10427 nucleotides （nt） in length excluding the poly（A） tail and was predicted to encode a polyprotein of 3340 amino acids （aa） with a calculated molecular weight of 379.7kD. SYSV was most closely related to onion yellow dwarf virus （OYDV） and resembled it in having a much larger P3 protein than other species in the genus. SYSV-O shared 93.2% nt identity to the published sequence of a welsh onion isolate of SYSV （SYSV-ZQ2）. The two isolates differed in NIb-CP cleavage site, that of SYSV-O was Q/V but Q/A in SYSV-ZQ2. Nucleotide identity of coat protein genes amongst all SYSV sequences ranged from 87. 3%-84.1%. Phylogenetic tree analysis based on the 3′-terminal nucleotide sequences of SYSV isolates confirmed the existence of four distinct groups that were partially related to geographical origin and host plant species.
出处
《病毒学报》
CAS
CSCD
北大核心
2007年第2期157-160,共4页
Chinese Journal of Virology
基金
浙江省自然科学基金重点项目(批准号:ZA0207)
国家自然科学基金项目(3020008)
国家杰出青年科学基金项目(30225031)
关键词
胡葱黄条病毒
洋葱分离物
基因组全序列
序列分析
系统进化树
Shallot yellow stripe virus
onion isolate
complete genome sequence
sequence comparison
phylogenetic tree analysis
