摘要
设计3对表达引物分别扩增侵染了百合无症病毒(lily symptomless virus,LSV),百合斑驳病毒(lily mottle virus,LMoV)和百合X病毒(lily virus X,LVX)的外壳蛋白基因,并在大肠杆菌BL21(DE3)plysS中原核表达。目的蛋白切胶后免疫小鼠制备多克隆抗体。Western blot检测表明3种病毒抗血清分别与目的蛋白起特异性反应,但与阴性对照无反应。3种病毒间无交叉血清学反应。对田间栽种的百合样品间接ELISA检测表明,LMoV和LSV均有发生,但没有检测到LVX。
The specific primers were designed to amplify the coat protein genes of lily symptomless virus （LSV, genus Carlavirus）, lily mottle virus（LMoV, genus Potyvirus） and lily virus X（LVX, genus Potexvirus） respectively. The plasmids containing CP genes were constructed and prokaryotic expressed in E.coli. The antisera specific to the CPs were subse- quently prepared. LSV and LMoV were detected from lily plants in Zhejiang province by indirect-ELISA, but LVX was not detected.
出处
《科技通报》
2007年第2期219-224,共6页
Bulletin of Science and Technology
基金
浙江省自然科学基金重点项目(ZA0207)
国家自然科学基金项目(30200008
30470080)
浙江省自然科学基金(M303018)
浙江省重大招标项目(030823010)
