禁食对小鼠杏仁核HAP1表达的影响

The Influence of Food Deprivation on the Expression of HAP1 in the Hypothalamic of Mice

目的探讨小鼠杏仁核中的亨廷顿蛋白相关蛋白HAP1(huntingtin associated protein 1,HAP1)的分布及是否与饥饿有关。方法采用半定量免疫印迹法(Western blotting)检测禁食过程小鼠杏仁核HAP1表达的变化。应用免疫组织化学染色法观察禁食对杏仁核HAP1表达的影响。结果连续禁食1日、2日、3日、4日的小鼠杏仁核的HAP1含量与正常进食的小鼠相比,呈逐渐增多的趋势。其HAP1阳性细胞的表达随禁食时间的延长显著增高。结论HAP1与饥饿有关,并有可能参与了食欲的调节。

Objective： To probe for if huntingtin associated protein 1 （HAP1） is involved in hungry. Methods： Western blotting was used to observe the effect of food-deprivation on expressing of HAP1 in the amygdala of the mouse Method of immunohistochemistry was used to determine the change of HAP1 in the amygdala. Results： It was detected by western blotting that the HAP1 expression in the amygdala increases when food-deprivation continued. By immunohistochemistry, HAP1 immunoreactivity was shown to enhanced in the amygdala. Conclusion： HAP1 might be involved in hungry and participate in appetite regulation.