流式细胞技术与传统试验在阵发性睡眠性血红蛋白尿诊断中的联合应用

Combinative Application of Flow Cytometry and Classical Lab of Hb in Treatment of PNH

目的分析应用流式细胞技术与传统试验相结合来诊断阵发性睡眠性血红蛋白尿的(PNH)价值。方法用流式细胞分析术对PNH患者的红细胞(RBC)及白细胞(WBC)进行膜糖蛋白CD55、CD59表达情况的检测,同时进行蔗糖溶血、酸化溶血(Ham)实验及尿含铁血黄素(Rous)实验。结果所有PNH患者CD55+、CD59+红细胞及白细胞所占比率均显著降低,庶糖溶血试验阳性者占100%、Ham实验阳性者占96%、Rous实验阳性者占91%,且患者溶血的程度与CD55+、CD59+细胞比率成反比。结论流式细胞技术结合传统试验相互补充、印证,可提高PNH的确诊率。

Objective To analyze the value of application with flow cytometry and classical lab of Hb in treatment of PNH. Methods The RBC, WBC, CD55, CD59, Ham and Rous of PNH patient were detected with combined lab tests. Results The CD55, CD59, RBC and WBC were decreased obviously and 100% sucrose hemolysis test, 96% Ham and 91% Rous those degrees were negative with CD55 and CD59. Conclusion This method combined with classical method might supplement to improve the diagnostic rate of PNH.