宫颈癌和宫颈上皮内瘤变中p53基因第5～8外显子突变的检测

The detection of p53(exon5～8)mutations in cervical cancer and cervical intraepithelial neoplasia

目的为探讨p53基因第5～8外显子突变与宫颈癌发生发展的关系。方法采用聚合酶链反应单链构象多态性(PCR-SSCP)分析方法对37例宫颈鳞癌(SCC)、16例宫颈腺癌(AUC)及45例宫颈上皮内瘤变(CIN)中p53基因第5～8外显子突变进行检测。结果SCC中p53基因突变率约为10．81%(4/37),2例位于外显子5,2例位于外显子7;AUC中p53基因突变率约为18．75% (3/16),其中2例位于外显子5,1例位于外显子7;CIN组中p53基因突变率约为4．44%(2/45),1例位于外显子5,1例位于外显子7;三者间p53基因突变率比较差异无显著意义(P＞0．05、P＞0．05、P＞0．05)。结论p53基因第5～8外显子突变可能不是宫颈癌发生的主要原因,p53基因突变仅与少数宫颈癌的发生有关。

Objective To investigate the relationship between p53 （exon5-8） mutations and oncogenesis of cervical cancer. Methods p53 （exon5-8） mutations was detected by polymerase chain reaction- single strand conformation polyrnorphism （PCR-SSCP） in 37 cases of squamous carcinoma of the cervix（SCC）, 16 cases of adenocarcinoma of uterine cervix（AUC） ,and 45 cases of cervical intraepithelial neoplasia（CIN）. Results The frequency of p53 （exon5-8） mutations was 10. 81%（4/37） in SCC , 2 cases were located in exon5 and 2 cases were situated in exonT; the frequency of p53 （exon5-8） mutations was 18. 75%（3/16） in AUC , 2 cases were situated in exon5 and 1 cases was located in exon7; while the rate of p53 （exon5-8） mutations was 4.44%（2/45） in CIN ,1 case was situated in exon5 and 1 case was situated in exon7; no significant difference can be showed between them（P〉0. 05）. Conclusion p53 （exon5-8） mutations might not be the major cause of oncogenesis of cervical cancer. The mutations of p53 only have correlation to oncogenesis of minority cervical cancer.