H-ras干扰性RNA对卵巢癌裸鼠皮下移植瘤CD44V6表达的影响

The effect of interfering RNA targeting H-ras gene to CD44V6 expression in xenografted tumor of ovarian cancer cells

目的应用重组质粒pshRNA/H-ras转染卵巢癌SKOV3荷瘤裸鼠,观察Ras信号传导通路中信号分子的变化。方法选用裸鼠20只建立卵巢癌裸鼠皮下成瘤模型,分别设立对照组和实验组,应用陡脉冲电转重组质粒pshRNA/H-ras于瘤体中,于治疗后21天处死裸鼠并取瘤体组织固定,免疫组织化学SP法检测CD44V6的表达。结果对照组细胞膜、浆中见变异型细胞黏附分子44(CD44V6)的阳性表达,而转染pshRNA/H-ras实验组CD44V6的表达显著下降,差异有统计学意义(P＜0．05)。结论H-ras的小干扰性RNA可下调与肿瘤转移相关基因的表达,对阻止肿瘤侵袭转移具有较好的研究价值。

Objective To transfer the short hairpin RNA recombinant plasmid targeting H-ras gene （pshRNA/H-ras） to tumor cells and detect the changes of signal molecules in signal transduction pathway. Methods The recombinant plasmids targeting H-ras gene coding sequence （pshRNA/H-ras） were constructed successfully. Tumor modeling were achieved in 20 nude mice and divided into two groups： experimental group injecting pshRNA/H-ras four times groups, the rest were used for negative control group. After 21 days, the CD44V6 expression were detected by IHC. Results From pathological observations, the expression of CD44V6 were positive in cell membrane and cytoplasm in control group. The introduction of siRNAs targeting H-ras was showed to specifically inhibit the expression of CD44V6 in experimental group, which has significant difference（P〈0. 05）. Conclusion Small interfering RNA of H-ras can modulate the expression about tumor gene related metastasis and have a valuable worth to inhibit tumor metastasis.