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H-ras干扰性RNA对卵巢癌裸鼠皮下移植瘤CD44V6表达的影响 被引量:2

The effect of interfering RNA targeting H-ras gene to CD44V6 expression in xenografted tumor of ovarian cancer cells
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摘要 目的应用重组质粒pshRNA/H-ras转染卵巢癌SKOV3荷瘤裸鼠,观察Ras信号传导通路中信号分子的变化。方法选用裸鼠20只建立卵巢癌裸鼠皮下成瘤模型,分别设立对照组和实验组,应用陡脉冲电转重组质粒pshRNA/H-ras于瘤体中,于治疗后21天处死裸鼠并取瘤体组织固定,免疫组织化学SP法检测CD44V6的表达。结果对照组细胞膜、浆中见变异型细胞黏附分子44(CD44V6)的阳性表达,而转染pshRNA/H-ras实验组CD44V6的表达显著下降,差异有统计学意义(P<0.05)。结论H-ras的小干扰性RNA可下调与肿瘤转移相关基因的表达,对阻止肿瘤侵袭转移具有较好的研究价值。 Objective To transfer the short hairpin RNA recombinant plasmid targeting H-ras gene (pshRNA/H-ras) to tumor cells and detect the changes of signal molecules in signal transduction pathway. Methods The recombinant plasmids targeting H-ras gene coding sequence (pshRNA/H-ras) were constructed successfully. Tumor modeling were achieved in 20 nude mice and divided into two groups: experimental group injecting pshRNA/H-ras four times groups, the rest were used for negative control group. After 21 days, the CD44V6 expression were detected by IHC. Results From pathological observations, the expression of CD44V6 were positive in cell membrane and cytoplasm in control group. The introduction of siRNAs targeting H-ras was showed to specifically inhibit the expression of CD44V6 in experimental group, which has significant difference(P〈0. 05). Conclusion Small interfering RNA of H-ras can modulate the expression about tumor gene related metastasis and have a valuable worth to inhibit tumor metastasis.
出处 《贵州医药》 CAS 2007年第3期212-214,共3页 Guizhou Medical Journal
关键词 RNA干扰 H—ras基因 卵巢上皮性癌 裸鼠皮下移植瘤 CD44V6 RNA interfering H-ras gene Ovarian epithelial carcinoma Xenografts in nude mice CD44V6
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  • 1Guo S,Kemphues KJ.Par-1,a gene required for establishing polarity in C.elegans embryos,encodes a putative Ser/Thr kinse that is asymmetrically distributed[J].Cell,1995,81:611-620.
  • 2Fire A,Xu S,Montgomery MK,et al.Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans[J].Nature,1998,391:806-811.
  • 3Salvi A,Arici B,De Petro G,et al.Small inteHering RNA urokinase siliencing inhibits a invasion and migration of human hepatocellular carcinoma cells[J].Mol Cancer Ther,2004,3(6):671-678.
  • 4Pardrige Pardridge WM.Intravenous,non-viral RNAi gene therapy of brain cancer[J].Expert Opin Biol Ther,2004.4(7):1 103-1 113.
  • 5Hang L,Yang N,Mohamed-Hadley A,et al.Vector-based RNAi,a novel tool for isoform-specific knock-down of VEGF and anti-angiogenesis gene therapy of cancer[J].Biochem Biophys Res.Commun,2003,303(4):1 169-1 178.
  • 6王萍玲,胡丽娜,张君,邓凯贤,黄爱龙.RNAi沉默卵巢癌耐药细胞株SKOV3/ADM内源H-ras基因表达对细胞增殖能力的影响[J].现代妇产科进展,2006,15(5):328-331. 被引量:4
  • 7王萍玲,胡丽娜,李聪,孙才新,米彦,王士彬,姚成果.能量可控陡脉冲联合H-ras干扰性RNA治疗卵巢癌裸鼠皮下移植瘤的实验研究[J].第三军医大学学报,2005,27(6):490-493. 被引量:4
  • 8Gong Yang,Jennifer Anne Thompson,Bingliang Fang,et al.Silencing of H-ras gene expression by retrovirus-mediateel siRNA decreases transformation efficiency and tumorgrowth in a model of human ovarian cancer[J].Oncogene,2003,22(36):5 694-5701.
  • 9Hofmann M,Rudy W,Gunthert H,et al.A link between ras and metastatic behavior of tumor cells:ras induces CD44 promoter activity and leads to low-level expression of metastasisspecific variants of CD44 in CREF cells[J].Cancer Res,1993,53(7):1 516-1 521.
  • 10Kogerman P,Sy MS,Gulp LA.Oncogene-dependent expression of CD44 in Balb/c 3T3 derivatives:c orrelation with metastatic competence[J].Clin Exp Matastasis,1996,14(1):73-82.

二级参考文献13

  • 1Okino M, Mohri H. Effects of a high-voltage electrical impulse and anticancer drug on in vivo growing tumors[J]. Jpn J Cancer Res, 1987, 78(12): 1319-1321.
  • 2Weaver J C. Electroporation: a general phenomenon for manipulating cells and tissues[J]. J Cell Biochem, 1993, 51(4): 426-435.
  • 3Tomoaki Goto, Toru Nishi, Takahiko Tamura, et al. Highly efficient electro-gene therapy of solid tumor by using an expression plasmid for the herpes simplex virus thymidine kinase gene[J]. Proc Natl Acad Sci U S A, 2000, 97(1): 354-359.
  • 4Takeshi Suzuki, Bo-Chul Shin, Keiko Fujikura, et al. Direct gene transfer into rat liver cells by in vivo electroporation[J]. FEBS Lett, 1998, 425: 436-440.
  • 5Gong Yang, Jennifer Anne Thompson, Bingliang Fang, et al. Silencing of H-ras gene expression by retrovirus-mediated siRNA decreases transformation efficiency and tumorgrowth in a model of human ovarian cancer[J]. Oncogene, 2003, 22(36): 5694-5701.
  • 6Guo S,Kemphues KJ.Par-1,a gene required for establishing polarity in C.elegans embryos,encodes a putative Ser/Thr kinase that is asymmetrically distributed[J].Cell,1995,81:611-620
  • 7Fire A,Xu S,Montgomery MK,et al.Potent and specific genetic interference by double-stranded RNA in caenorhabditis elegans[J].Nature,1998,391:806-811
  • 8Pardrige WM.Intravenous,non-viral RNAi gene therapy of brain cancer[J].Expert Opin Biol Ther,2004,4:1103-1113
  • 9Li K,Lin SY,Brunicardi FC,et al.Use of RNA interferce to target cyclin E-overexpressing hepatocellular carcinoma[J].Cancer Res,2003,63:3593-3597
  • 10Zhang L,Yang N,Mohamed-Hadley A,et al.Vector-based RNAi,a novel tool for isoform-specific knock-down of VEGF and anti-angiogenesis gene therapy of cancer[J].Biochem Biophys Res Commun,2003,303:1169-1178

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