survivin在非小细胞肺癌中的表达及其与bc1-2、p63表达的关系

Expression of Survivin and Its Relationship with Expression of bc1-2,p63 in Non Small Cell Lung Cancer

目的:探讨survivin在非小细胞肺癌组织(non small cell lung cancer,NSCLC)中的表达,及其与bc 1 2、p63蛋白表达的相关性。方法:应用二步法免疫组织化法,检测survivin、bc 1-2、p63蛋白在60例NSCLC组织和20例正常肺组织中的表达。结果:肺癌组织中的survivin蛋白阳性率(56．67%)明显高于正常肺组织15%),有显著性差异;(p＜0．05)Ⅲ期surviving蛋白阳性表达率72．73%(16/22)明显高于Ⅰ+Ⅱ期survivin47．37%(18/38)。有显著差异;(p＜0．05)survivin蛋白表达与患者年龄、病理类型、组织分化程度,淋巴结转移情况无关(P＞0．05)NSCLC组织bc 1-2蛋白表达阳性、阴性组中,survivin蛋白阳性表达率分别为66．67% (18/27)和48．48%(16/33),两者比较,差异有显著性(P＜0．05);p63蛋白表达阳性、阴性组中,survivin蛋白阳性表达率分别为53．33%(16/30)和60%(18/30),两者比较,差异有显著性(P＜0．05)survivin,蛋白与bc 1-2蛋白的表达呈正相关。survivin蛋白与p63蛋白的表达呈正相关。结论:survivin在NSCLC组织中表达上调,通过抑制细胞凋亡,在NSCLC的发生和发展中起到重要作用。survivin,bc 1-2与p63它们分别在肺癌发生发展过程中不同途径上抑制肺癌细胞的凋亡,对肺癌早期诊断有一定的意义。对3种蛋白进行联合检测,更有利于肺癌的早期诊断和判断肺癌的分化程度、临床分期、淋巴结是否转移及病人的预后。survivin与bc1-2及survivin与p63可能起协同作用,并可能会成为NSCLC基因治疗的新靶点。

Objective： To study the expression of Survivin in non small cell lung cancer （NSCLC） and its relationship with the expression of bcl-2, p63 proteins. Methods： Expression of the Survivin, bcl-2 and p63 proteins was evaluated by immunohistochemical polymer detection system method in 60 NSCLC tissues and 20 normal lung tissues. Results： The positive expression rates （56.67%）of Survivin with NSCLC was significantly higher than that （15% of normal lung tissue （P〈0.05）. The positive expression rates （ 72.73%）of Survivin in Stage Ⅲ were significantly higher than those in Stage Ⅰ ＋Ⅲ （47.37% （P〈0.05）. There was no correlation between Survivin and age, gender, pathological type, histological differentiation,lymph node metastases statius（P〉0.05）. Survivin positive cases were strongly associated with bcl-2 expression （66.67%versus 48.48%,P〈0.05 ） and p63 expression （53.33% versus 60%, P〈0.05 ）. The expreesoin of Bcl-2 was positively correlated with the expression of survivin. The expreesoin of p63 was positively correlated with the expression of survivin. Conclusions： Upregulation of Survivin expression in non small cell lung cancer suggests that Survivin may play an important role in the oncogenesis and progression of non small cell lung cancer by the inhibition of apoptosis. Survivin, bcl-2 andp63 inhibit apoptosis of tumor cells on the differet way during the pathogenesis of lung cancer, respectively. Determining three protein expressions simultaneously might be more helpful to making an early diagnosis of NSCLC, judging its differentiation degree, clinical stage and lymph node status, as well as the prognosis. They play a certain role in evaluating prognosis and early diagnosis of pulmonary carcinoma. Survivin may be identified as a new thera- peutic target, cooperating with Bcl-2and p63.