HLA-DRB1基因与湘西土家族人群肺结核的易感性分析

Study on the correlation between HLA-DR genes and susceptibility to pulmonary tuberculosis in a population of Tujia nationality from west part of Hunan province in China

目的:探讨HLA-DRB1基因多态性与湘西土家族人群肺结核的遗传关联性。方法:用病例与对照的研究方法,采用聚合酶链反应-序列特异性引物(PCR-SSP)技术,对69例湘西土家族人群肺结核患者和100例湘西土家族人群健康对照者HLA-DRB1等位基因进行分析。结果:与对照组相比,PTB病例组的DRB109基因频率增高(63.7%比14%Pc<0.0001RR=10.81),而DRB111基因频率显著低于对照组(11.6%比29%Pc<0.05)。结论:HLA-DRB109可能是湘西土家族人群肺结核的易感基因,HLA-DRB111可能为保护基因。

Objective：To analyze the HLA-DRB1 gene polymorphism and the hereditary association with pulmonary tuberculosis in a popualtion of Tujia nationality from west part of Hunan provence in China.Methods：Using case-control study,the analysis of the HLA- DRB1 gene sites in 69 tuberculosis cases in Tujia nationality from west part of Hunan province in China and 100 healthy controls in the same nationality from Jishou was performed by polymerase chain reaction-sequence specific primers （PCR-SSP） technique.Results：Compared with the control group,the frequency of DRB1 09 allele in pulmonary tuberculosis cases was strikingly higher than that in the healthy controls （63.7% vs 14%,Pc〈0.0001 ,RR=10.81）, but the DRB1 11 strkingly lower （11.6% vs 29%,Pc〈0.05）.Condusion：The results suggest that DRB1 09 allele is closely correlated to the incidence of pulmonary tuberculosis in this population of Tujia nationality from west part of Hunan province in China.It is also suggested that the expression of DRB1 11 allele may be associated with an antagonist effect in the pathogenesis of pulmonary tuberculosis in this population.