人和小鼠Dspp基因的克隆及其在成牙本质细胞中的表达检测

Cloning of DSPPs and Their in Situ Detection in Odontoblasts of Human and the Mouse

目的克隆人和小鼠牙本质涎磷蛋白(DSPP)基因片断,建立一种从分子水平检测成牙本质细胞分化的方法。方法制备特异性的人和小鼠Dspp基因片断的mRNA反义探针,再将其分别与人和小鼠具有成牙本质细胞的牙胚组织进行原位杂交,检测Dspp基因表达情况。结果本实验制备出的人和小鼠Dspp基因的mRNA反义探针在特定条件下能对各自的成牙本质细胞中Dspp基因的表达进行检测。结论本方法可以作为一种从分子水平检测人或小鼠成牙本质细胞分化的工具。

Objective To clone both haumna and mice DSPP cDNA fragments were cloned from the exon sequences of the genomic DAN with PCR method. Methods DIG labeled antisense mRNA probes were prepared for in situ detection of DSPP expression in odontoblasts of human and mice. Results Our results showed that the probes could specifically detect DSPP expression in odontoblasts of either human or mice respectively. Conclusions The method employed in this study could be used as molecular tools for examing the differentiation of odontoblasts in human and mice.