Cry1Ab蛋白在不同土壤中的吸附与解吸

ADSORPTION/DESORPTION OF CRY1AB CRYSTAL PROTEIN IN DIFFERENT SOILS

以转cry1Ab基因高粱为材料提取Cry1Ab蛋白,测定了Cry1Ab蛋白在6种土壤中的吸附与解吸,分析了Cry1Ab蛋白溶液浓度、土壤理化性质对Cry1Ab蛋白吸附与解吸的影响。结果表明,不同类型的土壤对Cry1Ab的吸附与解吸存在明显的差异,吸附量表现为红泥土>灰化土>青紫泥田>黄筋泥>黄松田>红砂土,解吸量表现为灰化土>青紫泥田>红砂土>黄筋泥>红泥土>黄松田;Cry1Ab蛋白溶液的浓度与吸附量和解吸量呈显著正相关,相关系数分别为0.86和0.99;土壤有机质、pH值与土壤吸附Cry1Ab蛋白的能力呈显著正相关,相关系数分别为0.83和0.82;土壤全氮、有效磷的含量与土壤吸附Cry1Ab蛋白呈正相关,土壤全氮、有效磷和速效钾的含量与解吸均呈负相关。土壤吸附、解吸Cry1Ab蛋白是加入Cry1Ab蛋白溶液的浓度及不同土壤的理化性质共同作用的结果。

Adsorption/desorption of Cry1Ab crystal protein extracted from crylAb-transgenie sorghum in six types of soils were determined, and effects of concentration of Cry1Ab protein in the solution and physical and chemical property of 6 types of soils on adsorption/desorption of the protein were studied. Results show that adsorption and desorption of Cry1Ab protein in the 6 soils were obviously different. Paddy soil from red clay was the highest in Cry1Ab adsorption, and followed by typic podzolie soil, blue purple clay soil, paddy soil from quaternary red clay, yellow loamy paddy soil and paddy soil derived from red sandstone in a decreasing order, while typic podzolie soil was found the highest in Cry1Ab desorption and followed by blue purple clay soil, paddy soil derived from red sandstone, paddy soil from quaternary red clay, paddy soil from red clay and yellow loamy paddy soil. A significant positive correlation was found between the concentration of Cry1Ab in the solution added and the adsorption or desorption of the protein by the soils, with coefficients being 0.86 and 0.99, respectively. Effects of organic matter content and pH of the soil on adsorption were positive and significant, with coefficients being 0.83 and 0.82, respectively. The correlation between the adsorption and the content of total nitrogen or readily available phosphorus was positive, while it was negative between the desorption and the content of total nitrogen, readily available phosphorus or readily available potash. Consequently the adsorption and desorption of Cry1Ab in the soils were controlled jointly by concentration of Cry1Ab protein and physical and chemical properties of the soil.