无精子症患者睾丸穿刺中评价支持细胞意义

The significance of evaluate sertoli cell in testicular fine needle aspiration from patient with azoospermia

目的评价无精子症患者睾丸穿刺中检测支持细胞指数的意义。方法采用Johnson评分法评价睾丸活检组织生精情况;采用瑞-姬染色,人工判读睾丸穿刺液涂片中各期生精细胞和支持细胞,并对其进行分类计数。结果睾丸活检组织随着Johnson评分降低,支持细胞形态结构越不规则;睾丸穿刺中生精正常组9例,支持细胞指数范围均在20%～40%之间;生精障碍组15例,其中11例支持细胞指数范围在80%～150%,4例支持细胞指数范围在30%以下;生精阻滞组9例,支持细胞指数范围均在150%～500%之间,1例为15%。结论生精正常组与生精障碍组、生精阻滞组的支持细胞指数差异明显,无精子症患者睾丸穿刺报告中支持细胞指数具有重要意义。

Objctive To evaluate the significance of analyzing sertoli cells in testicular fine needle aspiration from patient with azoospermia. Methods Spermatogenic function of testicular biopsy were evaluated by Johnson score method.Spermatogenic cells and sertoli cells were analyzed by visual interpretation with Wright-Giemsa staining and then were counted under the microscope. Results The lower of Johnson score of testicular biopsy, the more irregular of sertoli cell morphology;Statistical significance of difference of sertoli index was detected between normal spermatogenesis group and the two other groups （hypospermatogenesis and arrest spermatogenesis）. Conclusion Sertoli index has important significance in report of testicular fine needle aspiration of patient with azoospermia.