板栗疫病菌脱毒方法的比较研究

Comparison of methods to eliminate virus from chestnut blight fungus Cryphonectria parasitica

病毒与宿主相互作用中,存在宿主主动或被动摆脱病毒的机制。本研究用光照诱导-单孢分离、原生质体再生和菌丝尖端分离3种方法对携带低毒病毒的板栗疫病菌(Cryphonectria parasitica)的EP721、EP713和Euro7三个菌株的脱毒效率进行了比较。结果表明:经光照诱导-单孢分离后所有菌株均可获得脱毒菌株,而原生质体再生对EP721的脱毒效率最高,是继光照诱导-单孢分离脱毒法之后另一种新的有效的脱毒方法,特别适用于不产孢的真菌脱毒。

In virus-host interaction, there are mechanisms for a host to eliminate virus either actively or passively. Efficiency of light induced sporulation-single conidial spore isolation, spheroplast regeneration, and meristemic culture in eliminating or escaping virus from the hypovirus-infected Cryphonectria parasitica strains EP721, EP713 and Euro7 was evaluated. Single spore isolation was effective in all treatments though with varied efficiency and spheroplast regeneration was also an efficient method to obtain virus-free colonies from infected strains. The latter may be particularly useful in obtaining the virus-free culture in case where a virus-infected fungus does not produce spores.