侵染月季的李坏死环斑病毒的检测及CP基因序列分析

Detection and sequencing of CP gene of Prunus necrotic ring spot virus in rose

Rose leaves with virus symptoms(mosaic,ring spot and line pattern) were detected by DAS-ELISA and RT-PCR.The results showed Prunus necrotic ring spot virus(PNRSV) infection in the leaves.CP gene of PNRSV isolated from Yunnan province was cloned and sequenced.Analysis of sequence showed that CP gene of PNRSV-yunnan contained 683 nucleotides encoding 165 aminoacids(EMBL accession number: AY684271).Nucleotide similarity of CP gene was more than 98% between PNRSV-yunnan and group I isolates,and less than 95% between PNRSV-yunnan and group II and III isolates.According to the results,PNRSV-yunnan was identified as a member of group I.

Rose leaves with virus symptoms （mosaic, ring spot and line pattern） were detected by DAS-ELISA and RT-PCR. The results showed Prunus necrotic ring spot virus （PNRSV） infection in the leaves. CP gene of PNRSV isolated from Yunnan province was cloned and sequenced. Analysis of sequence showed that CP gene of PNRSV-yunnan contained 683 nucleotides encoding 165 aminoacids （EMBL accession number： AY684271）. Nucleotide similarity of CP gene was more than 98% between PNRSV-yunnan and group Ⅰ isolates, and less than 95% between PNRSV-yunnan and group Ⅱ and Ⅲ isolates. According to the results, PNRSV-yunnan was identified as a member of group Ⅰ.