摘要
通过表面接枝改性法对上转换发光材料Na[Y0.57Yb0.39Er0.04]F4进行表面修饰羧基的研究,并对影响因素如反应时间、缓冲溶液以及修饰剂的加入量进行了系统的实验分析,解决了上转换发光材料作为荧光标记物使用时无法直接共价固定生物活性分子的问题.实验结果表明,当反应时间为4h,缓冲溶液为pH=9.0,0.50mol·L-1的PB缓冲溶液,缓冲溶液的使用量为50mL,修饰剂的加入量为氨基化材料质量的12倍时,上转换发光材料表面成功修饰羧基的量达到最大.
The surface of an up-conversion luminescence material was modified with carboxyl group in the way of grafting modification. The influencing factors including reaction time, buffer solution and finishing agent＇s mass were investigated by experiment. This modification method has resolved the problem that when an up-conversion luminescence material is used as fluorescence labeling, it cannot covalently fix biological activity molecules directly. The experimental results show that when the reaction time is 4 h, the PB buffer solution is pH = 9.0 and 0.50 mol· L^-1, agent to original materials is activity carboxyl group. the volume of adding the buffer solution is 50 mL, and the mass ratio of finishing 12, the surface of an up-conversion luminescence material is mostly modified with
出处
《北京科技大学学报》
EI
CAS
CSCD
北大核心
2007年第3期298-301,共4页
Journal of University of Science and Technology Beijing
基金
国家自然科学基金资助项目(No.50372006
No.20273007
No.20407003)
关键词
发光材料
上转换
表面修饰
羧基
luminescence materials
up-conversation
surface modification
carboxyl group
