应用圆二色光谱研究电场对辣根过氧化物酶二级结构的影响

Study on the Effect of Electric Field on the Secondary Structure of HRP by Circular Dichroism

辣根过氧化物酶（HRP）经不同强度的电场处理，用圆二色光谱研究了电场对辣根过氧化物酶二级结构的影响。结果表明：在1．0～6．0kV·cm^-1范围，不同强度的电场对辣根过氧化物酶的α-螺旋、β-折叠、β-转角及无规卷曲相对含量的影响程度不同。与对照组相比，处理组的α-螺旋、α-转角和无规卷曲含量降低，降幅范围分别为0．3％～11．4%，1．7％～15．3％和0．9％～20．4%;β-折叠含量增加，增幅范围为0．9％～82．7％。电场作用具有使辣根过氧化物酶的二级结构中α-螺旋、β-转角和无规卷曲向β-折叠转化的趋势。研究结果对解释电场处理种子生物效应具有重要意义。

After the HRP was treated with different strength of electric field, the effect of electric field on the secondary structure of HRP was studied by circular dichroism. The results show that different electric field strength ranging from 1.0 to 6.0 kV·cm^-1 has a different effect on the relative contents of α-helix , β-sheet,β-turn and random coil of the HRP. As compared with the contrast, the relative contents of α-helix,β-turn and random coil decreased by 0. 3%-11.4%, 1.7%-15.3% and 0. 9%- 20.4% respectively, whileβ-sheet content increased by 0. 9%-82.7%. The electric treatment tends to transform the α-helix, β- turn and random coil into β-sheet for the HRP. The result of this study has important meaning to explain the biological effect of electric treatment seeds.