摘要
目的研究硒锗联合作用对染氟大鼠血清、肝、肾中脂质过氧化物(MDA)水平和谷胱甘肽过氧化物酶(GSH-Px)活性及钙、镁的影响。方法给SD大鼠饮用含NaF(100mg/L)的蒸馏水90d,同时每天灌胃给予Na2seO30.1mg/kg和(或)锗-13210mg/kg。对大鼠血清、肝、肾组织中的GSH-Px活性、MDA、钙、镁水平进行测定。结果硒锗联合增加了GSH-Px活性,降低MDA水平;硒和(或)锗对氟诱导的大鼠血清、肝脏、肾脏中的钙水平降低具有明显抑制作用,硒锗联合对氟诱导的大鼠肝脏、肾脏中钙抑制作用强于单纯给予硒或锗;硒和(或)锗对氟诱导的大鼠血清、肾脏中的镁水平降低具有明显抑制作用,硒锗联合对氟诱导的大鼠肝脏的镁水平降低具有明显抑制作用。结论硒锗联合对氟毒性具有明显的拮抗作用,其机制与抗脂质过氧化作用有关;硒锗联合对氟诱导的大鼠钙镁变化的影响具有协同作用。
Objective To study the effect of Se-Ge agent on lipid peroxide (MDA), the activity of glutathione peroxidase(GSH-Px) and major elements such as calcium and magnesium level in fluorosis rats. Methods Sprague-Dawley rats were given distilled water containing NaF(100 mg/L), followed by administration of Na3SeO3 (0.1 mg/kg) and/or Ge-132 (10 mg/kg) via gastric gavage. The activity of GSH-Px and the content of MDA, Ca, Mg in rats' serum, liver, kidneys were detected. Results Se-Ge agent enhanced GSH-Px activity,while lowering MDA level. Na2SeO3 and Ge-132 had powerful antagonistic effects on fluoride-induced decrease of Ca, Mg content in serum, liver, kidney of rats, and the effect was more potent when the two agents were used together. Conclusions The combination of Se-Ge agents markedly antagonizes fluoride-induced toxicity, via a mechanism of antagonizing lipid peroxidation. Se and Ge have synergistic effect on changes of major elements.
出处
《中国地方病学杂志》
CAS
CSCD
北大核心
2007年第2期137-139,共3页
Chinese Jouranl of Endemiology
基金
宁夏自然科学基金资助项目(NZ0570)
关键词
氟
硒
锗
抗氧化酶
Fluoride
Selenium
Germanium
Antioxidase