硒锗联合对氟致大鼠血清和肝肾抗氧化酶及钙镁元素变化的影响

Effect of selenium-germeniun agent on antioxidase and major dements in fluorosis rats

目的研究硒锗联合作用对染氟大鼠血清、肝、肾中脂质过氧化物（MDA）水平和谷胱甘肽过氧化物酶（GSH-Px）活性及钙、镁的影响。方法给SD大鼠饮用含NaF（100mg／L）的蒸馏水90d，同时每天灌胃给予Na2seO30．1mg／kg和（或）锗-13210mg／kg。对大鼠血清、肝、肾组织中的GSH-Px活性、MDA、钙、镁水平进行测定。结果硒锗联合增加了GSH-Px活性，降低MDA水平；硒和（或）锗对氟诱导的大鼠血清、肝脏、肾脏中的钙水平降低具有明显抑制作用，硒锗联合对氟诱导的大鼠肝脏、肾脏中钙抑制作用强于单纯给予硒或锗；硒和（或）锗对氟诱导的大鼠血清、肾脏中的镁水平降低具有明显抑制作用，硒锗联合对氟诱导的大鼠肝脏的镁水平降低具有明显抑制作用。结论硒锗联合对氟毒性具有明显的拮抗作用，其机制与抗脂质过氧化作用有关；硒锗联合对氟诱导的大鼠钙镁变化的影响具有协同作用。

Objective To study the effect of Se-Ge agent on lipid peroxide （MDA）, the activity of glutathione peroxidase（GSH-Px） and major elements such as calcium and magnesium level in fluorosis rats. Methods Sprague-Dawley rats were given distilled water containing NaF（100 mg/L）, followed by administration of Na3SeO3 （0.1 mg/kg） and/or Ge-132 （10 mg/kg） via gastric gavage. The activity of GSH-Px and the content of MDA, Ca, Mg in rats＇ serum, liver, kidneys were detected. Results Se-Ge agent enhanced GSH-Px activity,while lowering MDA level. Na2SeO3 and Ge-132 had powerful antagonistic effects on fluoride-induced decrease of Ca, Mg content in serum, liver, kidney of rats, and the effect was more potent when the two agents were used together. Conclusions The combination of Se-Ge agents markedly antagonizes fluoride-induced toxicity, via a mechanism of antagonizing lipid peroxidation. Se and Ge have synergistic effect on changes of major elements.