摘要
研究杜仲叶中绿原酸含量的检测方法。利用绿原酸和铝离子的络合显色反应，采用可见分光光度法在波长530nm处测定杜仲叶中绿原酸量。绿原酸质量浓度在1.7×10^-4～1．0×10^-2～g·L^-1之间线性关系良好，线性相关系数为0．9995。秋、夏叶中加标回收率分别为98．0％，101．0％。该方法简便、实用、准确。
The determination of chlorogenic acid in Eucommia Ulmodies leaves was inuestigated. The complexation reaction of aluminum （Al^3＋ ） with chlorogenic acid and visible spectrophotometry was used for the determination at 530 nm. The calibration curve is linear in the range of 1.7 × 10^-4 ～ 1.0 × 10^-2g · L^-1 concentration for chlorogenic acid. The corresponding regression equation was Y = 0. 003 92 X - 0. 006 7 ,r =0. 999 5. The spliked recovery rates are 98% and 101% for the leaves in autumn and summer, respectively. The results showed that this method is simple, practical and accurate.
出处
《生物加工过程》
CAS
CSCD
2007年第1期71-73,共3页
Chinese Journal of Bioprocess Engineering
关键词
杜仲叶
绿原酸
可见分光光度法
Eucommia Ulmodies leaves
chlorogenic acid
visible spectrophotometry
